The present invention relates to a primer for PCR obtained by, directly or through inosine as a linker, linking a complementary nucleotide sequence or a complementary nucleotide sequence including a mis-matched nucleotide sequence to the 5′-terminal of a forward or reverse primer; and to a PCR method including a step of mixing a nucleic acid template in a PCR composition including the primer and then performing PCR on the mixture. The primer for PCR of the present invention includes a complementary nucleotide sequence or a mis-matched nucleotide sequence in a complementary nucleotide sequence, which is linked to the 5′-terminal thereof directly or via a linker, thereby lowering the sensitivity increase due to the increase in amplification products and reducing non-specifically occurring reactions in PCR.

The present invention relates to a primer for PCR obtained by, directly or through inosine as a linker, linking a complementary nucleotide sequence or a complementary nucleotide sequence including a mis-matched nucleotide sequence to the 5′-terminal of a forward or reverse primer; and to a PCR method including a step of mixing a nucleic acid template in a PCR composition including the primer and then performing PCR on the mixture. The primer for PCR of the present invention includes a complementary nucleotide sequence or a mis-matched nucleotide sequence in a complementary nucleotide sequence, which is linked to the 5′-terminal thereof directly or via a linker, thereby lowering the sensitivity increase due to the increase in amplification products and reducing non-specifically occurring reactions in PCR.

This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.

This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.

The invention relates to methods for pairwise sequencing of a polynucleotide template which result in the sequential determination of nucleotide sequence in two distinct and separate regions of the polynucleotide template.

The invention relates to methods for pairwise sequencing of a polynucleotide template which result in the sequential determination of nucleotide sequence in two distinct and separate regions of the polynucleotide template.

Compounds useful as nucleic acid probes are disclosed. In some embodiments, the compounds have the following structure (I) or a stereoisomer, tautomer or salt thereof, wherein M, L.sup.1a, L.sup.2, L.sup.8, L.sup.1b, L.sup.3, L.sup.5, L.sup.6, L.sup.7, L.sup.4, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sub.a, R.sub.b, R.sub.c, R.sub.d, m, n, q, and w are as defined herein. Methods associated with preparation and use of such compounds are also provided.

Compounds useful as nucleic acid probes are disclosed. In some embodiments, the compounds have the following structure (I) or a stereoisomer, tautomer or salt thereof, wherein M, L.sup.1a, L.sup.2, L.sup.8, L.sup.1b, L.sup.3, L.sup.5, L.sup.6, L.sup.7, L.sup.4, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sub.a, R.sub.b, R.sub.c, R.sub.d, m, n, q, and w are as defined herein. Methods associated with preparation and use of such compounds are also provided.

The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.

The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.