Disclosed herein is a transmembrane nanosensor device comprising a lipid conjugated DNA tweezer, and methods of using the same.

Disclosed herein is a transmembrane nanosensor device comprising a lipid conjugated DNA tweezer, and methods of using the same.

The present invention provides a method of predicting whether a pulmonary nodule in a subject is benign or non-small cell lung cancer, comprising obtaining the results of an assay that measures an expression level of miR205-5p in a plasma sample from the subject; obtaining the results of an assay that measures an expression level of miR126 in a plasma sample from the subject; obtaining the results of an assay that provides a size of the pulmonary nodule in the subject; and calculating a probability value based on the combination of the expression levels of miR205-5p and miR126, and the size of the pulmonary nodule, wherein if the probability value exceeds a specified threshold, the pulmonary nodule is predicted as non-small cell lung cancer.

The present invention relates to a method for predicting susceptibility to sorafenib treatment by using an SULF2 gene, and a composition for treatment of sorafenib-resistant cancer using the SULF2 expression inhibition. The method for predicting susceptibility to sorafenib treatment by using the SULF2 gene according to the present invention can enable achievement of an optimal therapeutic effect by administering a drug suitable for cancer patients, and the composition for treatment of sorafenib-resistant cancer using the SULF2 inhibition has a very excellent anticancer treatment effect.

The invention relates to methods of detecting and/or quantifying analytes in a sample, as well as methods of detecting mutations and/or polymorphisms in nucleic acid molecules. The methods include: providing at least one carrier nucleic acid molecule comprising at least one single-stranded region; providing at least one detection element comprising: at least one fluorophore, at least one fluorescence quencher that quenches spectroscopic detection of the fluorophore; at least one analyte-binding moiety; and at least one nucleic acid moiety that binds to a single stranded region on the carrier nucleic acid molecule; wherein the detection element is configured such that in the absence of the analyte the fluorophore is quenched by the fluorescence quencher and upon analyte binding to the analyte-binding moiety fluorescence is restored; binding these with an analyte to form a complex; translocating the complex through a nanopore via voltage-driven translocation and monitoring time-dependent current response; irradiating the nanopore with radiation that excites the fluorophore and monitoring radiation emissions of the fluorophore over time; and comparing the signals from time-dependent current response and emission over time.

The invention relates to methods of detecting and/or quantifying analytes in a sample, as well as methods of detecting mutations and/or polymorphisms in nucleic acid molecules. The methods include: providing at least one carrier nucleic acid molecule comprising at least one single-stranded region; providing at least one detection element comprising: at least one fluorophore, at least one fluorescence quencher that quenches spectroscopic detection of the fluorophore; at least one analyte-binding moiety; and at least one nucleic acid moiety that binds to a single stranded region on the carrier nucleic acid molecule; wherein the detection element is configured such that in the absence of the analyte the fluorophore is quenched by the fluorescence quencher and upon analyte binding to the analyte-binding moiety fluorescence is restored; binding these with an analyte to form a complex; translocating the complex through a nanopore via voltage-driven translocation and monitoring time-dependent current response; irradiating the nanopore with radiation that excites the fluorophore and monitoring radiation emissions of the fluorophore over time; and comparing the signals from time-dependent current response and emission over time.

Provided is a method for identifying whether an RNA molecule has a 2′-O-methylation modification on a nucleotide, said method comprising: (1) contacting the RNA molecule with a ribonuclease Rnase R; and (2) detecting whether the RNA molecule is degraded or detecting hydrolysis termination positions after degradation. If the RNA molecule is degraded, this indicates that the RNA molecule does not have a 2′-O-methylation modification on a 3′ terminal nucleotide, and if hydrolysis terminates at a same site on multiple random broken fragments, this indicates that the RNA molecule has a 2′-O-methylation modification on the nucleotide at the position immediately preceding the termination site. Also provided are applications of the method for screening for a disease diagnosis target and confirming whether a subject has a 2′-O-methylation modification-related disease.

Provided is a method for identifying whether an RNA molecule has a 2′-O-methylation modification on a nucleotide, said method comprising: (1) contacting the RNA molecule with a ribonuclease Rnase R; and (2) detecting whether the RNA molecule is degraded or detecting hydrolysis termination positions after degradation. If the RNA molecule is degraded, this indicates that the RNA molecule does not have a 2′-O-methylation modification on a 3′ terminal nucleotide, and if hydrolysis terminates at a same site on multiple random broken fragments, this indicates that the RNA molecule has a 2′-O-methylation modification on the nucleotide at the position immediately preceding the termination site. Also provided are applications of the method for screening for a disease diagnosis target and confirming whether a subject has a 2′-O-methylation modification-related disease.

The present invention may provide a small RNA detection sensor comprising: at one end thereof, a first sensing region comprising nucleotides having a sequence complementary to target small RNA; and a PCR-capable region that is coupled to the first sensing region, the small RNA detection sensor to synthesize a replication region complementary to the PCR-capable region by a DNA polymerase by using the target small RNA as a primer, and amplify the PCR-capable region and the replication region.

The present invention may provide a small RNA detection sensor comprising: at one end thereof, a first sensing region comprising nucleotides having a sequence complementary to target small RNA; and a PCR-capable region that is coupled to the first sensing region, the small RNA detection sensor to synthesize a replication region complementary to the PCR-capable region by a DNA polymerase by using the target small RNA as a primer, and amplify the PCR-capable region and the replication region.