The present invention provides a system and method for automatic nucleic acid extraction and qualitative analysis. The system comprises a magnetic rotary mixer which comprises a plurality of magnetic rods for generating magnetism, configured to be retractable from the magnetic rotary mixer; a plurality of spin shaft for mounting tips, and the plurality of magnetic rods extend therein; an auto stage comprises a plate holder, which allows a plate place thereon; a mixer holder to hold the magnetic rotary mixer over the plate holder; and a heat plate, disposed under the plate holder for heating the plate. The present invention provides an automated high-throughput nucleic acid extraction and qualitative diagnosis with high efficiency and high accuracy, which is easy to interpret for operators, and realize that nucleic acid extraction and molecular detection can be completed at one time in a single device.

The present invention provides a system and method for automatic nucleic acid extraction and qualitative analysis. The system comprises a magnetic rotary mixer which comprises a plurality of magnetic rods for generating magnetism, configured to be retractable from the magnetic rotary mixer; a plurality of spin shaft for mounting tips, and the plurality of magnetic rods extend therein; an auto stage comprises a plate holder, which allows a plate place thereon; a mixer holder to hold the magnetic rotary mixer over the plate holder; and a heat plate, disposed under the plate holder for heating the plate. The present invention provides an automated high-throughput nucleic acid extraction and qualitative diagnosis with high efficiency and high accuracy, which is easy to interpret for operators, and realize that nucleic acid extraction and molecular detection can be completed at one time in a single device.

The present invention relates to a composition for diagnosing whether someone has been infected with a novel coronavirus (SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2), a kit comprising the composition, and a method for diagnosing whether someone has been infected with the novel coronavirus by using same. Particularly, the present invention relates to a composition for diagnosing whether someone has been infected with a novel coronavirus, comprising a nucleic acid oligomer capable of specifically amplifying by targeting a leader sequence most abundantly present in cells infected with the novel coronavirus, a kit comprising the composition, and a method for diagnosing whether someone has been infected with the novel coronavirus by using same.

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

The present invention discloses a primer set of LAMP-LFD detection for detecting a leaf curl virus of Melia azedaeach L. and a detection method, and belongs to the field of detection of crop diseases. The primer set of LAMP-LFD visual detection includes five primers: CLSV-F3, CLSV-B3, CLSV-FIP, CLSV-BIP and CLSV-LB and one probe primer: CLSV-Pb. The present invention also provides a visual detection method for a leaf curl virus of Melia azedaeach L., which is a combination of a loop-mediated isothermal amplification method and a lateral flow dipstick to establish an LAMP-LFD method to detect CLSV, so that the results of LAMP amplification detection are more obvious and intuitive. The present invention has the characteristics of high specificity, high sensitivity and simple operation, and is suitable for substrate and field use.

The present invention discloses a primer set of LAMP-LFD detection for detecting a leaf curl virus of Melia azedaeach L. and a detection method, and belongs to the field of detection of crop diseases. The primer set of LAMP-LFD visual detection includes five primers: CLSV-F3, CLSV-B3, CLSV-FIP, CLSV-BIP and CLSV-LB and one probe primer: CLSV-Pb. The present invention also provides a visual detection method for a leaf curl virus of Melia azedaeach L., which is a combination of a loop-mediated isothermal amplification method and a lateral flow dipstick to establish an LAMP-LFD method to detect CLSV, so that the results of LAMP amplification detection are more obvious and intuitive. The present invention has the characteristics of high specificity, high sensitivity and simple operation, and is suitable for substrate and field use.

A method for seeding and amplifying target nucleic acids derived from a sample in a cluster at a site on a surface of a substrate includes retaining at least a portion of the target nucleic acids in an inactive form that cannot seed to provide a relatively low concentration of active form target nucleic acids available for seeding. As the active form target nucleic acids seed on the surface of the substrate, they may be amplified. Because the concentration of active form target nucleic acids is low, the likelihood is low that a second active form target nucleic acid will seed at the same site within the same cluster before the first active form target nucleic acid is sufficiently amplified to dominate. Accordingly, the likelihood that the cluster will pass filters is increased relative to traditional seeding and amplification methods employing a higher concentration of active form target nucleic acids.