DNA is sequenced by (a) independently sequencing first and second strands of a dsDNA to obtain corresponding first and second sequences; and (b) combining the first and second sequences to generate a consensus sequence of the dsDNA. By independently sequencing first and second strands the error probability of the consensus sequence approximates a multiplication of those of the first and second sequences.

DNA is sequenced by (a) independently sequencing first and second strands of a dsDNA to obtain corresponding first and second sequences; and (b) combining the first and second sequences to generate a consensus sequence of the dsDNA. By independently sequencing first and second strands the error probability of the consensus sequence approximates a multiplication of those of the first and second sequences.

Provided includes methods, compositions and systems for single molecule seeding and amplification on a flow cell. In some embodiments, nucleic acids are isothermally seeded and amplified on a flow cell comprising multiple binding areas (e.g., pads), resulting in an ensemble of substantially the same amplified molecules on each of the binding areas.

Provided includes methods, compositions and systems for single molecule seeding and amplification on a flow cell. In some embodiments, nucleic acids are isothermally seeded and amplified on a flow cell comprising multiple binding areas (e.g., pads), resulting in an ensemble of substantially the same amplified molecules on each of the binding areas.

A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.

A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.

The present invention relates to a method of detecting a target nucleic acid on the basis of rolling circle amplification (RCA), and more specifically, to a method of detecting a target nucleic acid, the method in which a target nucleic acid (a nucleic acid having a target nucleic acid sequence), when present, forms a circular template with a template for performing an amplification reaction, wherein during the amplification reaction, a restriction enzyme is added to further induce a new RCA reaction, thus increasing the reaction rate and sensitivity, and to an RCA composition for implementing the method. The method of detecting a target nucleic acid according to the present invention, by detecting a barcode sequence predefined according to the type of the target nucleic acid, enables multiple detections of the presence of the target nucleic acid without sequencing, is inexpensive for not using costly enzymes, such as CRISPR, can detect barcode sequences, and can utilize various existing nucleic acid detection systems, and thus, can be useful in the detection of gene mutations.

The present invention relates to a method of detecting a target nucleic acid on the basis of rolling circle amplification (RCA), and more specifically, to a method of detecting a target nucleic acid, the method in which a target nucleic acid (a nucleic acid having a target nucleic acid sequence), when present, forms a circular template with a template for performing an amplification reaction, wherein during the amplification reaction, a restriction enzyme is added to further induce a new RCA reaction, thus increasing the reaction rate and sensitivity, and to an RCA composition for implementing the method. The method of detecting a target nucleic acid according to the present invention, by detecting a barcode sequence predefined according to the type of the target nucleic acid, enables multiple detections of the presence of the target nucleic acid without sequencing, is inexpensive for not using costly enzymes, such as CRISPR, can detect barcode sequences, and can utilize various existing nucleic acid detection systems, and thus, can be useful in the detection of gene mutations.

A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.

A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.