The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention relates to a drum stencil printing system for reliably and automatically printing materials on the inside of a concave surface. The drum stencil printing system includes a pliable drum stencil secured in place by a stencil spring. As the printhead applies radial pressure to the drum stencil, the portion of the stencil in contact with the printhead is deflected to make contact with the container sidewall. When the printhead rotates, the portion of the stencil contacting the container sidewall also rotates to match the nozzle of the printhead. After printing is complete, the printhead retracts, and the pliable stencil is returned to its undeflected position, breaking contact between the stencil and the container, and protecting the printed material from being rubbed or worn off of the container.

The present invention relates to a drum stencil printing system for reliably and automatically printing materials on the inside of a concave surface. The drum stencil printing system includes a pliable drum stencil secured in place by a stencil spring. As the printhead applies radial pressure to the drum stencil, the portion of the stencil in contact with the printhead is deflected to make contact with the container sidewall. When the printhead rotates, the portion of the stencil contacting the container sidewall also rotates to match the nozzle of the printhead. After printing is complete, the printhead retracts, and the pliable stencil is returned to its undeflected position, breaking contact between the stencil and the container, and protecting the printed material from being rubbed or worn off of the container.

Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.