Disclosed are novel nitrilated psilocybin derivative compounds and pharmaceutical and recreational drug formulations containing the same. The compounds may be produced by reacting a reactant psilocybin derivative with a nitrile-group containing compound.

Phenotypically modified bioengineered microbial spores are provided. The microbial spores may be used in various spore-based technologies such as probiotics, biomaterials and vaccines.

Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.

The present disclosure relates to a Bacillus amyloliquefaciens CJ14-6 strain isolated from traditional meju, a preparation method for soybean koji using the same, and a soybean koji prepared by the preparation method. The preparation method for soybean koji includes: soaking soybeans in water or adding waster to soybeans and steaming the soaked soybeans; and inoculating a Bacillus amyloliquefaciens CJ14-6 strain into the steamed soybeans, fermenting the steamed soybeans, and drying the fermented soybeans to prepare a soybean koji.

The present disclosure provides a microbial agent YF, including four strains: Bacillus sonorensis B-2, Bacillus paralicheniformis B-26, Bacillus sonorensis N-16, and Bacillus pumilus N-17. The four strains were all deposited in the China General Microbiological Culture Collection Center (CGMCC) on Mar. 5, 2020. The Bacillus sonorensis B-2 has a deposit accession number of CGMCC NO. 19454, the Bacillus paralicheniformis B-26 has a deposit accession number of CGMCC NO. 19449, the Bacillus sonorensis N-16 has a deposit accession number of CGMCC NO. 19448, and the Bacillus pumilus N-17 has a deposit accession number of CGMCC NO. 19450. The microbial agent provided by the present disclosure can effectively prevent stem rot of corn in saline-alkali land, improve the quality of saline-alkali land, and significantly promote the growth of corn.

Stress is any demand placed on the brain or physical body, thereby leading to anxiety and depression and it is a common phenomenon experienced by humans and animals. Stress, anxiety and depression often leads to serious mental ill health. People with anxiety disorders respond to certain things or situations with fear and dread and display physical signs such as a pounding heart and sweating. Often, people lose their willpower, concentration and interest in day to day activities, are drained of energy and can even become devoid of any emotion.

It is documented that low activity of certain neurotransmitters can result in anxiety, chronic stress, depression, difficulty in concentrating, memory problems, muscle pain, headaches, insomnia and sleep problems. Therefore, if the level of these neurotransmitters is raised, the problem of stress, anxiety and depression can be cured. The invention describes a formulation to naturally increase the levels of certain neurotransmitters in the body, thereby reducing stress, anxiety and depression.

The present invention overcomes the problem of processes yielding poor quantities of poly-gamma-glutamic-acid (γ-PGA) by providing a process and a novel medium for synthesis of γ-PGA in the presence of Bacillus paralicheniformis MCC 196. Wherein, the novel medium comprises tomato waste alone for cost effective and greener production of γ-PGA with yield of 40 g/L. In another process, non-sterile fermentation employs the use of a medium high in sugar content. Wherein, sucrose is used in concentrations up to 50% to obtain more than 280 g/L of poly gamma glutamic acid. The disclosed process is scalable and provides immense economic advantages.

The present disclosure relates to the technical field of Gastrodia elata planting, in particular to an organic planting method of G. elata, including the process of mycelia colonized material preparation: cutting a branch of Quercus acutissima into bars; cutting out fish scale openings on the bars to obtain bars with the fish scale openings; successively soaking the bars with the fish scale openings in boiling water and a growth-promoting solution to obtain bars after growth promotion treatment; and putting Amillariella mellea spawns and an inoculation substrate into each fish scale opening of the bars after the growth promotion treatment to obtain a mycelia colonized material. The growth-promoting solution and the inoculation substrate are prepared by treatment of fresh G. elata stalks with Bacillus pumilus and hot-dip extraction treatment in ethanol.

A biological indicator for determining the efficacy of an oxidative sterilization process, and its methods of use. The biological indicator comprises a set of microbial spores, at least one fluorescent sensor protein, and a culture medium, the fluorescent sensor protein being capable of yielding an optically detectable signal when the fluorescent sensor protein is not in a denatured state due to the oxidative sterilization process, and a different optically detectable signal when the fluorescent sensor protein is in a denatured state after the oxidative sterilization process.

The subject invention provides methods and compositions for replacing chemical surfactants for use in a wide variety of industrial applications. More specifically, the subject invention provides for the production of multi-functional biological surface-active compositions having one or more precise functional characteristics based on the desired use.