The present invention provides a method for producing a basic L-amino acid, for example, L-ornithine L-citrulline, L-arginine, L-histidine, and L-lysine by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress a gene encoding a protein having L-methionine/branched chain amino acid exporter activity.

The invention relates to the validation of decontamination processes and in particular to new surrogate organisms and mixtures of said microorganisms used for validating the decontamination processes.

The present invention relates to the field of biocontrol of plant pathogenic fungi, particularly to bacterial strains effective in treating and/or preventing plant diseases associated with phytopathogenic fungi and/or oomycetes; preparations, lysates and extracts thereof, compositions comprising same and use thereof.

Variant Erwinia chrysanthemi L-asparaginases with reduced L-glutaminase activity and enhanced in vivo circulation are described as are fusion proteins containing an L-asparaginase and three tandem soluble domains of TRAIL for use in the treatment of cancers such as acute lymphoblastic leukemia and acute myeloid leukemia.

Provided herein are methods for integrating a gene of interest into a chromosome of a host cell. In some embodiments, the methods include introducing into a host cell a first plasmid comprising a transposase coding sequence and a donor sequence, which includes a selectable marker coding sequence flanked by a first and a second lox site and is itself flanked by inverted repeats recognized by the transposase. Following transposase-mediated chromosomal integration of the donor sequence into the host cell, a second plasmid is introduced, which comprises the gene of interest and a second selectable marker coding sequence, both flanked by a first and a second lox site. The gene of interest is chromosomally integrated into the host cell by recombinase-mediated cassette exchange (RMCE) between the donor sequence and the second plasmid via Cre-/cuc recombination. Further provided herein are host cells, vectors, and methods of producing a product related thereto.

This disclosure generally relates to engineered recombinant bacterial cells producing enzymatic compositions for up-cycling plastics, biomass waste, or co-mixtures of plastics and biomass waste. The disclosure also relates to dual enzyme compositions produced by the recombinant bacterial cells for upcycling plastic materials and/or biomass waste, and methods for upcycling plastics, OHD-treated substrates and/or biomass waste.

A method for enhancing the degradation performance of lignin-degrading bacteria Erwinia sp. QL-Z3 and a culture medium for culturing the bacteria. The rate of degradation of an Erwinia sp. QL-Z3 strain to lignin is optimized from 14.23% before optimization to 25.01%. Under the conditions that the initial pH value of the culture medium is 8, the nitrogen source is NH.sub.4NO.sub.3, and the addition amount of lignin is 3 g/L, the activity of an LiP enzyme can be optimized to 371.00 U/L, which is 3.53 times that before optimization. When the initial pH value of the culture medium is 9.5, the nitrogen source is NH.sub.4NO.sub.3, and the concentration of lignin is 2.5 g/L, the activity of MnP and Lac enzymes can be optimized to 839.50 U/L and 219.00 U/L, respectively, which are 3.18 and 2.84 times that before optimization.