Provided herein are methods for integrating a gene of interest into a chromosome of a host cell. In some embodiments, the methods include introducing into a host cell a first plasmid comprising a transposase coding sequence and a donor sequence, which includes a selectable marker coding sequence flanked by a first and a second lox site and is itself flanked by inverted repeats recognized by the transposase. Following transposase-mediated chromosomal integration of the donor sequence into the host cell, a second plasmid is introduced, which comprises the gene of interest and a second selectable marker coding sequence, both flanked by a first and a second lox site. The gene of interest is chromosomally integrated into the host cell by recombinase-mediated cassette exchange (RMCE) between the donor sequence and the second plasmid via Cre-/cuc recombination. Further provided herein are host cells, vectors, and methods of producing a product related thereto.

Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC13085 and TIC13087. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC13085 and TIC13087 pesticidal proteins are also provided.

The present invention relates to an endophyte strain isolated from a plant of the Poaceae family, wherein said endophyte is a strain of Erwinia gerundensis which provides bioprotection and/or biofertilizer phenotypes to plants into which it is inoculated. The present invention also discloses plants infected with the endophyte and related methods.

The present invention relates to the field of biocontrol of plant pathogenic fungi, particularly to bacterial strains effective in treating and/or preventing plant diseases associated with phytopathogenic fungi and/or oomycetes, preparations, lysates and extracts thereof and compositions comprising same, and methods of use thereof.

Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC13085 and TIC13087. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC13085 and TIC13087 pesticidal proteins are also provided.

The present invention provides a method for producing a basic L-amino acid such as, for example, L-ornithine L-citrulline, L-arginine, L-histidine, and L-lysine by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress a gene encoding a protein having L-methionine/branched-chain amino acid exporter activity.

The invention relates to the validation of decontamination processes and in particular to a composition comprising new indicator organisms and a dye, said composition being used for validating the decontamination processes.

The invention relates to the validation of decontamination processes and in particular to new surrogate organisms and mixtures of said microorganisms used for validating the decontamination processes.

Variant Erwinia chrysanthemi L-asparaginases with reduced L-glutaminase activity and enhanced in vivo circulation are described as are fusion proteins containing an L-asparaginase and three tandem soluble domains of TRAIL for use in the treatment of cancers such as acute lymphoblastic leukemia and acute myeloid leukemia.

A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing in a culture medium a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, and collecting the L-amino acid from the culture medium and/or cells of the bacterium, wherein the bacterium has been modified to have one or more of the following modifications: (A) modification of reducing the activity of a BudA protein; (B) modification of reducing the activity of a BudB protein; (C) modification of reducing the activity of a BudC protein; (D) modification of reducing the activity of a PAJ_3461 protein; (E) modification of reducing the activity of a PAJ_3462 protein; and (F) modification of reducing the activity of a PAJ_3463 protein.