Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.

The present invention relates to a recombinant microorganism which is capable of simultaneously fermenting at least two sugars in a lignocellulosic saccharified liquid, and also capable of generating diol.

Disclosed are an enhanced efficient nitrogen-fixing composite microbial system added with non-nitrogen-fixing bacteria and application thereof, belonging to the technical field of agricultural microorganisms. The present disclosure provides enhanced efficient nitrogen-fixing bacteria, including at least one selected from a group of Klebsiella MNAZ1050, Citrobacter MNAZ1397 and Pseudomonas MNAZ228; also, the disclosed enhanced efficient nitrogen-fixing composite microbial system includes nitrogen-fixing bacteria and non-nitrogen-fixing bacteria, where the nitrogen-fixing bacteria includes at least one of the above three nitrogen-fixing bacteria, and the non-nitrogen-fixing bacteria includes at least one of Acinetobacter ACZLY512 and Kluyvera AZ981.

The present disclosure provides farmers a new platform for supplying nitrogen to their crops, which is based upon sustainable, biologically fixed nitrogen. The taught platform enables improved yield consistency across all cultivated acreage, irrespective of: weather, environment, or soil conditions. As a result of the increased yield consistency enabled by the taught disclosure, farmers have an increased degree of predictability for yield across each acre they plant, which was not possible with the synthetic nitrogen delivery paradigm of years past.

The present invention concerns a new method for the production of 1,3-propanediol comprising culturing a recombinant microorganism converting glycerol into 1,3-propanediol and overexpressing the hcpR and/or frdX gene on a medium comprising glycerine. A recombinant microorganism for the production of 1,3 propanediol from glycerol, wherein said microorganism converts glycerol into 1,3-propanediol and 10 overexpresses the hcpR and/or the frdX gene.

The present disclosure provides consortia of microbes that are functionally optimized for nitrogen fixation and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The microbes within the consortium differ in nutrient utilization, temporal occupation, oxygen adaptability, and/or spatial occupation, which enables the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plant's life cycle) manner. The present disclosure also provides methods of creating a synthetic composition of microbes and methods of using compositions of microbes to fix atmospheric nitrogen and deliver such to a crop.

The present disclosure provides a Klebsiella pneumoniae Y7-3 with a deposit number of CCTCC NO: M2019851. The strain can degrade corn stover into acetic acid, ethanol, and hydrogen, and can further metabolize into acetic acid, ethanol, 1,3-propanediol, lactic acid, and hydrogen.

Systems for plant culture include a chamber featuring one or more walls enclosing a spatial volume internal to the chamber, where the one or more walls include a surface for supporting a plant within the enclosed spatial volume, a gas delivery apparatus with at least one gas source, a nutrient delivery apparatus with a reservoir, a sampling apparatus connected to a port formed in the one or more walls, and a controller configured so that during operation of the system, the controller activates the nutrient delivery apparatus to deliver an aqueous growth medium to the plant, and activates the gas delivery apparatus to deliver into the enclosed spatial volume a mixture of isotopically-substituted gases. Also provided are methods of use of the system for measuring nitrogen in a plant and for identifying microbes capable of providing fixed nitrogen to a plant.

The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.