The invention relates to acetylcholine-producing microorganisms for use in the prevention and/or treatment of intestinal diseases, and/or reduction of risks of intestinal diseases, and/or improvement of intestinal health as well as promoting healthy gut flora. The acetylcholine-producing microorganisms may be provided as a pharmaceutical dosage form or as additive to functional food or food supplemental products. Also encompassed is a method for the production of acetylcholine by use of Lactobacilli. Further the invention refers to microbially produced acetylcholine for use in the treatment and/or prevention of intestinal diseases.

Isolates strains of gastrointestinal bacteria from wolves are provided for use as probiotics. In some embodiments, the isolated strains are for use as probiotics in canine subjects such as domestic dogs. In some embodiments, the isolated strains may be used to treat or prevent intestinal dysbiosis in the subject. Also provided are compositions comprising at least one isolated strain of wolf probiotic bacteria and related methods for making same.

Provided are Saccharomyces cerevisiae SPC-SNU 70-1 (KCTC 12776BP) which is novel natural Korean lactic acid bacteria isolated from traditional Korean nuruk, and Lactobacillus brevis SPC-SNU 70-2 (KCTC 12777BP), Lactobacillus curvatus SPC-SNU 70-3 (KCTC 12778BP) and Lactobacillus sanfranciscensis SPC-SNU 70-4 (KCTC 12779BP) which are novel natural Korean yeasts isolated from traditional Korean nuruk.

Compositions are provided for providing support to companion animals affected by Inflammatory Bowel Disease (IBD) and/or Irritable Bowel Syndrome (IBS). In some embodiments, the composition comprises at least one isolated strain of wolf probiotic bacteria and at least one isolated strain of canine probiotic bacteria. In some embodiments, the composition further comprises at least one prebiotic. Also provided are related methods for preparing a composition and for treating IBS and/or IBD in a subject.

Provided herein are fermented beverages comprising symbiotic microbial communities, and methods of production thereof.

An object is to provide lactic acid bacteria that can be applicable to feed, fertilizers, live bacterial preparations, probiotics, etc. The lactic acid bacteria are identified and deposited under the accession numbers of NITE (National Institute of Technology and Evaluation) P-02313, NITE P-02314, NITE P-02490, NITE P-02491, and NITE P-02492. In addition, another object is to provide feed, fertilizers and live bacterial preparations containing the lactic acid bacterium/bacteria of the invention. According to studies, it is confirmed that the lactic acid bacteria are proliferated in animals including human, plants, etc. without being destroyed. According to studies, it is also confirmed that the lactic acid bacteria are proliferated in water, soil, etc. without being destroyed. Therefore, the lactic acid bacteria can be widely applicable in feed, fertilizers, live bacterial preparations, etc.

An object is to provide lactic acid bacteria that can be applicable to feed, fertilizers, live bacterial preparations, probiotics, etc. The lactic acid bacteria are identified and deposited under the accession numbers of NITE (National Institute of Technology and Evaluation) P-02313, NITE P-02314, NITE P-02490, NITE P-02491, and NITE P-02492. In addition, another object is to provide feed, fertilizers and live bacterial preparations containing the lactic acid bacterium/bacteria of the invention. According to studies, it is confirmed that the lactic acid bacteria are proliferated in animals including human, plants, etc. without being destroyed. According to studies, it is also confirmed that the lactic acid bacteria are proliferated in water, soil, etc. without being destroyed. Therefore, the lactic acid bacteria can be widely applicable in feed, fertilizers, live bacterial preparations, etc.

Provided herein are fermented beverages comprising symbiotic microbial communities, and methods of production thereof.

The disclosure provides methods of making a tetracycline inducible expression system in a cell. The methods include providing the cell with a first nucleic acid sequence comprising a first promoter operably linked to a tetracycline repressor gene coding sequence, providing the cell with a second nucleic acid sequence comprising a second promoter operably linked to a coding sequence of a gene of interest wherein the second promoter is modified to include one or more tetracycline repressor protein binding sites, and determining the expression of the gene of interest in the presence or absence of tetracycline. The disclosure further provides nucleic acid sequences, vectors and cells including the tetracycline inducible modified promoter.

Provided herein are fermented beverages comprising symbiotic microbial communities, and methods of production thereof.