The present invention comprises a novel method to engineer soil bacteria to produce powerful chlorinated auxins. While chlorinated auxins were only found in few plant species, this technology will allow the construction of soil bacterial strains capable of producing chlorinated derivatives of indole-3-acetic acid (IAA). A select halogenase can be expressed in soil bacteria by inserting it into the genome or through an expression vector. The engineered strains can then be applied to any plants to promote growth, thus having promising applications in agriculture.

The present invention provides for a Pseudomonas cell is able to grow in a medium with xylose or galactose as a sole carbon source with a growth rate of equal to or higher than 0.10 h.sup.−1. The present invention provides for methods and compositions relating to an engineered Pseudomonas putida KT2440 utilizing a non-native carbon source, such as galactose or xylose or both.

Disclosed in the present disclosure is a method for preparing (S)-1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid and derivatives thereof, comprising: taking a racemate of a compound represented by Formula (I) or a racemate of a salt of the compound represented by Formula (I) as a substrate, and making a R-isomer of the compound represented by Formula (I) in the substrate react under the catalysis of oxidative dehydrogenase to generate imino acid represented by formula (II); and converting the imino acid represented by Formula (II) into an S-isomer of the compound represented by Formula (I) in the presence of pipecolic acid reductase and a coenzyme capable of supplying hydrogen anions. The present disclosure is featured by mild reaction condition, strong stereoselectivity, high reaction efficiency, high conversion rate, etc.

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Provided is a method for producing 2-pyrone-4,6-dicarboxylic acid (PDC) by culturing a microorganism that produces PDC. The present invention provides a method of producing PDC by culturing a microorganism that produces 2-pyrone-4,6-dicarboxylic acid (PDC), wherein the method comprises: dissolving the starting substance for production of PDC in a buffer solution that contains no alkali metals, and adjusting the pH of a culture solution with a buffer solution that contains no alkali metals.

The present disclosure relates to a non-naturally occurring enzyme that includes a first polypeptide that catalyzes the hydrolysis of a polyester to produce mono-(2-hydroxyethyl) terephthalate (MHET), a second polypeptide that catalyzes the cleavage of MHET to produce at least one of terephthalic acid or ethylene glycol, and a third polypeptide that links the first polypeptide with the second polypeptide.

Provided herein are methods for integrating a gene of interest into a chromosome of a host cell. In some embodiments, the methods include introducing into a host cell a first plasmid comprising a transposase coding sequence and a donor sequence, which includes a selectable marker coding sequence flanked by a first and a second lox site and is itself flanked by inverted repeats recognized by the transposase. Following transposase-mediated chromosomal integration of the donor sequence into the host cell, a second plasmid is introduced, which comprises the gene of interest and a second selectable marker coding sequence, both flanked by a first and a second lox site. The gene of interest is chromosomally integrated into the host cell by recombinase-mediated cassette exchange (RMCE) between the donor sequence and the second plasmid via Cre-/cuc recombination. Further provided herein are host cells, vectors, and methods of producing a product related thereto.

Methods for bioremediation of environmental media contaminated with at least one perchlorate compound. A Pseudomonas consortium of P. putida strain B, P. putida strain E, and P. fluorescens strain G was provided to contaminated water, soil, etc. under conditions to result in bioremediated water, soil, etc. In embodiments, the method is used ex-situ, e.g., in a reactor vessel, or is used in-situ.

The present disclosure relates to a polypeptide having an acyltransferase activity or a microorganism including the same; a composition for preparing N-acetyl-L-methionine, the composition including the polypeptide or microorganism; and a method of preparing N-acetyl-L-methionine using the polypeptide or microorganism. Further, the present disclosure relates to a polynucleotide encoding the polypeptide and an expression vector including the polynucleotide. Since the microorganism including a novel acyltransferase according to the present disclosure has enhanced acyltransferase activity, this microorganism can be efficiently used for producing N-acetyl-L-methionine by acetylating L-methionine.

The present invention pertains to a method for producing high value-added compounds from polyethylene terephthalate. More specifically, the present invention demonstrates that a monomeric terephthalic acid obtained from the chemical hydrolysis of polyethylene terephthalate can be converted to high value-added aromatic compounds and aromatic-derived compounds, and ethylene glycol, which is another monomer of polyethylene terephthalate, can be converted to glycolic acid, which is a cosmetic material. The present invention is characterized by recycling polyethylene terephthalate waste into high value-added compounds.

The present invention refers to the strain CECT8538 of Pseudomonas putida and mutants thereof, and the use of said strain as a pesticide in controlling plant diseases caused by fungi and bacteria. Further aspects of the invention relate to methods for preparing pesticidal compositions comprising said strain. Finally, the invention relates to a method for controlling various plant diseases caused by fungi and bacteria in a plant, comprising treating the plant and/or seed thereof or substrate used for growing said plant with the strain CECT8538 of P. putida or a composition including it.