An object of the present invention is to provide an enzyme showing random transesterification ability, which is suitable for food applications. Provided is a random transesterification lipase having high heat resistance in oils and fats.

The present invention relates to Candida strains comprising a mutation or deletion in the first and/or second XYL2 allele which can be used for producing one or more sugar alcohols from a lignocellulosic feedstock. The preferred sugar alcohol is xylitol.

A long-chain composition has at least one long-chain alkane selected from the group consisting of C9-18 linear or branched alkanes and at least one long-chain carboxylic acid selected from the group consisting of C9-18 linear or branched, saturated or unsaturated aliphatic monocarboxylic acids. The mass ratio of the long-chain alkane to the long-chain carboxylic acid ranges from 1:1 to 40:1. The long-chain composition has a higher fermentation degree or higher substrate utilization rate and the like, when used as a starting material in the production of long-chain dibasic acids via fermentation.

The invention discloses a yeast and lactic acid bacteria combination, comprising saccharomyces and lactic acid bacteria; wherein the saccharomyces comprises Candida ethanolica B-JJ1, and the lactic acid bacteria comprise at least one of Lentilactobacillus buchneri B-JR1, Lactobacillus paracasei B-JR2, Lactobacillus zeae B-JR4, Lactobacillus plantarum B-JR5 and Lactobacillus chiayiensis B-JR6. The yeast and lactic acid bacteria combination is obtained by the processes of inoculum extraction, strain inoculation, combined bacteria optimization and combined bacteria domestication. The fermentation of the yeast and lactic acid bacteria combination provided by the invention can be adopted to prepare bio-enzyme preparation. And the prepared bio-enzyme preparation can be adopted to prepare natural, green, additive-free preservatives with strong antioxidant properties that maintain the activity of plant and animal cells.

A traditional Chinese medicine polysaccharide extract freeze-drying protective agent, a direct-to-vat starter and preparation methods thereof are disclosed, which belong to the technical field of feed starter. The compound traditional Chinese medicine polysaccharide extract freeze-drying protective agent includes Poria cocos polysaccharide extract, Atractylodes macrocephala polysaccharide extract, Radix codonopsis polysaccharide extract, L-tyrosine, α-cyclodextrin and polyethyleneimine. Its application in the freeze-drying protection of direct-to-vat starter of aquatic fermentation feed can reduce the damage to the cells caused by freeze-drying, improve the survival rate of the cells in the starter after freeze-drying, effectively prolong the storage period of the starter, and ensure that the fermentation activity remains at a high level during storage.

A method for producing cis-unsaturated fatty acid includes the operations below. (i) An oil-water mixture is provided, wherein the oil-water mixture includes 1 to 10 parts by weight of oil and 1 part by weight of water. (ii) 0.002 to 0.5 parts by weight of a recombinant Candida rugosa lipase 1 (rCRL1) is added into the oil-water mixture. (iii) The oil-water mixture is emulsified. (iv) The emulsified oil-water mixture is hydrolyzed and fatty acid is generated. (v) Oil-water is separated at a temperature of 55° C. to 65° C. and an oil phase layer is extracted. (vi) The cooling and filtering step is performed to obtain cis-unsaturated fatty acid.

An object of the present invention is to obtain a fermentative yeast having a highly efficient ethanol production without introducing a foreign gene. A further object is to obtain a fermentative yeast that is resistant to proliferation inhibitors such as organic acids, which prevent the growth of the fermentative yeast. Yeast having improved ethanol production ability was generated by introducing transaldolase and alcohol dehydrogenase gene by self-cloning to Meyerozyma guilliermondii that can produce ethanol effectively from pentose and hexose obtained by breeding. This fermentative yeast is deposited to NITE Patent Microorganisms Depositary under the accession number NITE ABP-01976.

Disclosed herein is a process for producing a recombinant Candida cell, which involves genetically engineering a parent Candida cell using a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas)(CRISPR/Cas) system. A recombinant Candida cell obtained using the process and a method for producing D-lactic acid from a biomass using the recombinant Candida cell are also disclosed.

The present disclosure is directed to methods of making non-ionic, amphiphilic surfactants from 5-hydroxymethyl-2-furfural, furan-2,5-dimethanol and bis-2,5-dihydroxymethyltetrahydrofurans and the novel compounds that are made from those methods.

The present invention relates to a synthetic promoter capable of controlling the expression of a target gene at various locations in the genome of an acid-resistant strain, and more particularly to a synthetic promoter including a core promoter derived from an acid-resistant strain and an upstream activating sequence (UAS) element serving as an enhancer. When the present invention is applied to a variety of genetic and metabolic engineering techniques for acid-resistant yeast, various metabolic networks can be configured as desired while controlling the expression level of the target gene, so a method of producing various metabolites using acid-resistant yeast is provided, and the cost of producing the metabolites can be greatly reduced depending on the properties of the acid-resistant yeast.