The present disclosure is related to the fields of biology, molecular biology, genetics, microbial fermentation, alcohol production and the like. The present compositions and methods relate to yeast strains comprising genetic modifications that results in modified yeast strains thereof comprising enhanced stress tolerance. Certain embodiments of the disclosure are therefore related to compositions and methods for increasing the efficiency of alcohol production using such modified yeast strains in fermentation reactions/processes.

The invention concerns a strain of Saccharomyces bayanus subsp. uvarum identified as SERIUS and deposited at the DBVPG with deposit number 36P. The invention further concerns the use of the strain of Saccharomyces bayanus subsp. uvarum identified as SERIUS (DBVPG 36P) as a food inoculate in the production of foods obtained through alcoholic fermentation, and a selection method for yeast strains of the group Saccharomyces adapted to alcoholic fermentation, particularly of grape, consisting of an isolation step for such yeasts from flowers.

The present disclosure relates to automated multi-module instruments, compositions and methods for performing nucleic acid-guided nuclease editing; specifically, methods, instruments, systems, and nucleic acids synthetic cassettes that improve the efficiency of gene editing using CRISPR enzymes in diploid cells—either on both chromosomes or selectively in one chromosome—without incurring loss of heterozygosity (LOH) and its often deleterious effects.

Methods of and system for growing and maintaining an optimized/ideal active yeast solution in the yeast tank and fermenter tank during the fermentation filling cycle are provided. A new yeast stage tank is used between the yeast tank and the fermenter tank allowing yeast to rapidly produce a huge amount of active young yeast cells for a fermenter during the filling period. A measurable and useful controlling factor, % DT/% Yeast by weight ratio (or “food” to yeast ratio), is used (e.g., % DT=glucose), which offers information on the health status of the yeast. The controlling factor is used to control the status of the yeast throughout the entire process.

The present invention is directed to compositions comprising hydrolase-secreting bacteria and fermenting microorganisms and use thereof, such as for fermentative production of ethanol.

Methods of and system for growing and maintaining an optimized/ideal active yeast solution in the yeast tank and fermenter tank during the fermentation filling cycle are provided. A new yeast stage tank is used between the yeast tank and the fermenter tank allowing yeast to rapidly produce a huge amount of active young yeast cells for a fermenter during the filling period. A measurable and useful controlling factor, % DT/% Yeast by weight ratio (or “food” to yeast ratio), is used (e.g., % DT=glucose), which offers information on the health status of the yeast. The controlling factor is used to control the status of the yeast throughout the entire process.

The present invention relates to a composition for transmuting a radioactive substance into a non-radioactive substance using complex microorganisms and a method for preparing the composition.

Methods of and system for growing and maintaining an optimized/ideal active yeast solution in the yeast tank and fermenter tank during the fermentation filling cycle are provided. A new yeast stage tank is used between the yeast tank and the fermenter tank allowing yeast to rapidly produce a huge amount of active young yeast cells for a fermenter during the filling period. A measurable and useful controlling factor, % DT/% Yeast by weight ratio (or “food” to yeast ratio), is used (e.g., % DT=glucose), which offers information on the health status of the yeast. The controlling factor is used to control the status of the yeast throughout the entire process.

Described are compositions and methods relating to modified yeast that over-express the ribonucleotide reductase inhibitor, HUG1. The yeast produces an increased amount of alcohol compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate in an undesirable end product.

The subject invention provides methods for producing phospholipids using yeasts not previously known to produce high concentrations of such phospholipids. In particular, Wickerhamomyces anomalus is cultivated in a specially-tailored nutrient medium and under cultivation conditions such that the yeast unnaturally produces high concentrations of phospholipids that resemble human cardiolipin and/or precursors thereof in structure and/or function. Yeast culture compositions are also provided, comprising yeast cells, growth medium, and high concentrations of phospholipids.