Culturing S. glucoliberatum PABB004 under conditions effective for the S. glucoliberatum PABB004 to secrete simple sugars into culture medium. In one or more embodiments, the conditions include a pH of 6.0 to 8.5. In some cases, the culture can include a second organism. A co-culture includes S. glucoliberatum PABB004 and a second organism, wherein the co-culture has a pH of 6.0 or greater. In one or more embodiments, the second organism is selected to produce a product of interest such as, for example, ethanol.

The present disclosure relates to the fields of biotechnology, molecular biology and genetic engineering. In particular, the present disclosure relates to a genetically modified alga comprising a recombinant cytochrome c6 gene, methods of producing the same and applications thereof. The present disclosure also relates to a codon optimised nucleic acid sequence encoding a cytochrome c6 polypeptide, expression cassette, vectors and host cell thereof. In an embodiment, the present disclosure also relates to a method of increasing biomass and photosynthetic efficiency of algae.

Disclosed is a mutant strain having the ability to produce polyhydroxybutyrate. The novel strain has a significantly high growth rate and an improved ability to produce PHB compared to existing PHB-producing cyanobacterial strains. Therefore, the novel strain is suitable for use in the production of PHB and the development of various products using PHB. In addition, the novel strain is useful as a photosynthetic strain for developing a PHB production process using industrial flue gas due to its ability to produce PHB from only CO.sub.2 without any additional organic carbon source. Also disclosed is a method for producing polyhydroxybutyrate using the mutant strain.

Provided is a polypeptide tag. The amino acid sequence of the polypeptide tag is Xaa1Xaa2Xaa3PHDYNXaa4Xaa5Xaa6 (SEQ ID NO: 37), wherein in the formula, Xaa1, Xaa2, Xaa3, Xaa4, Xaa5, and Xaa6 are each independently an amino acid or none. The polypeptide tag is used for labeling a target protein. In a second aspect, provided is a polypeptide fusion protein, comprising the following two structures: (1) any polypeptide tag according to the first aspect, and (2) a target protein connected to the polypeptide tag. Also provided are an in vitro cell-free protein synthesis system and an application thereof in in vitro protein synthesis. By constructing the polypeptide tag and a target protein as a fusion protein, the expression of the labeled target protein can be effectively increased without removing the polypeptide tag.

A genetically engineered microorganism for the production of a cannabinoid is described. The genetically engineered microorganism comprises at least one nucleic acid molecule encoding at least one cannabinoid biosynthetic pathway enzyme. The disclosure also relates to methods for producing a cannabinoid using a genetically engineered microorganism.

Disclosed is a method for culturing Haematococcus pluvialis containing a large amount of astaxanthin. According to one embodiment, the method includes culturing Haematococcus pluvialis under autotrophic conditions to prepare a culture containing cysts in which astaxanthin is accumulated and adding a nitrogen source to the culture to induce germination of the cysts.

Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Further, disclosed are examples of incorporation of these higher concentration OCFA products into food for human and non-human animal consumption.

The present invention relates to a novel Chlamydomonas strain with an improved oil generation function, the strain of the present invention having useful mycological characteristics as a strain that provides a useful substance, such as a vegetable oil, in a microalga, as the strain has a fast cell growth speed and an excellent lipid generation function compared to conventional strains. In particular, the present invention can provide a vegetable oil with improved stability and a longer preservation period by containing, in a cell, a large amount of antioxidant pigments such as lutein and zeaxanthin, and can, thereby, be usefully used in industries such as food, medicine, cosmetics, etc., which utilize a vegetable oil.

A material with a scaffold comprising a series of at least partially spaced fibers and gas vesicles locates between fibers. The gas vesicles comprise external anchoring modules that are effective to anchor the gas vesicles to the fibers.

The present disclosure is in the technical field of synthetic biology and metabolic engineering. The disclosure provides engineered viable bacteria. In particular, the disclosure provides viable bacteria with reduced cell wall biosynthesis additionally modified for production of glycosylated product. The disclosure further provides methods of generating viable bacteria and uses thereof. Furthermore, the disclosure in the technical field of fermentation of metabolically engineered microorganisms producing glycosylated product.