C12Y102/99004

Compositions and methods for converting methanol into hydrogen peroxide and carbon dioxide

The present invention provides for a method for producing hydrogen peroxide comprising: (a) contacting a methanol with a methanol oxidase bound with a flavin adenine dinucleotide (FAD) cofactor, such that the methanol is oxidized into a formaldehyde and the FAD cofactor is reduced into FADH.sub.2; (b) contacting the formaldehyde with the methanol oxidase or a formate oxidase bound with a FAD cofactor, such that the formaldehyde is oxidized into a formate and the FAD is reduced into FADH.sub.2; and (c) contacting oxygen with one or more of the FADH.sub.2 to produce hydrogen peroxide and oxidize FADH.sub.2 into FAD. The present invention also provides for a fusion protein comprising any two or all of methanol oxidase, formate oxidase, and formaldehyde dismutase.