The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production. Provided are engineered microorganisms capable of producing fatty dicarboxylic acids and products expressed by such microorganisms. Also provided are biological methods for producing fatty dicarboxylic acids.

Provided is a method of producing at least one omega-hydroxyl fatty acid, the method comprising: (a) contacting at least one alkane with at least one recombinant yeast cell in an aqueous medium, wherein the yeast cell is capable of oxidising the alkane to the corresponding omega-hydroxyl fatty acid and the yeast cell comprises a reduced fatty acid degradation capacity.

Provided is a method of producing at least one omega-hydroxyl fatty acid, the method comprising: (a) contacting at least one alkane with at least one recombinant yeast cell in an aqueous medium, wherein the yeast cell is capable of oxidising the alkane to the corresponding omega-hydroxyl fatty acid and the yeast cell comprises a reduced fatty acid degradation capacity.

The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production. Provided are engineered microorganisms capable of producing fatty dicarboxylic acids and products expressed by such microorganisms. Also provided are biological methods for producing fatty dicarboxylic acids.

A microorganism is cultivated in the presence of a carbon source. The microorganism is a transformed microorganism having an introduced fadH gene encoding 2,4-dienoyl-CoA reductase. That is, the transformed microorganism has an introduced foreign gene encoding a protein having an amino acid sequence of SEQ ID NO: 1 or an introduced foreign gene encoding a protein having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 1. The carbon source contains an oil containing unsaturated fatty acids as constituents or free unsaturated fatty acids.