A method of producing electrical power includes: a cathode having a porphyrin precursor attached to a substrate, and having a first enzyme, wherein the first enzyme reduces oxygen; an anode having a first region of an anode substrate and having a gold nanoparticle composition located thereon, and having a second region of the anode substrate having an enzyme composition located thereon, wherein the enzyme composition includes a second enzyme, wherein the first region and second region are separate regions; and a neutral fuel liquid in contact with the anode and cathode, the neutral fuel liquid having a neutral pH and a fuel reagent; and operating the fuel cell to produce electrical power with the neutral fuel liquid having the neutral pH and the fuel reagent.

The present invention addresses the problem of providing a novel protein crosslinking method. In the present invention, a crosslinking reaction is accelerated by causing both an oxidoreductase such as a laccase and a protein deamidase such as a protein glutaminase to act on a substrate protein.

Aspects of the present disclosure are drawn to methods of improving the expression of secreted cuproenzymes from host cells by manipulating the expression level of one or more proteins involved in copper transport in the host cell, e.g., membrane-bound copper transporting ATPases and soluble copper transporters. The present disclosure also provides compositions containing such improved host cells as well as products derived from the improved host cells that contain one or more cuproenzymes of interest.

A method of producing electrical power includes: a cathode having a porphyrin precursor attached to a substrate, and having a first enzyme, wherein the first enzyme reduces oxygen; an anode having a first region of an anode substrate and having a gold nanoparticle composition located thereon, and having a second region of the anode substrate having an enzyme composition located thereon, wherein the enzyme composition includes a second enzyme, wherein the first region and second region are separate regions; and a neutral fuel liquid in contact with the anode and cathode, the neutral fuel liquid having a neutral pH and a fuel reagent; and operating the fuel cell to produce electrical power with the neutral fuel liquid having the neutral pH and the fuel reagent.

The invention relates to a biocell (1) with a biofuel reservoir intended to be brought into contact with a liquid medium and with a fluid medium comprising an oxidant. Said biocell comprises a first electrochemical cell having: an anode (5) comprising a first enzyme capable of catalyzing the oxidation of the biofuel;—a cathode (7) comprising a second enzyme capable of catalyzing the reduction of the oxidant; and—a separating and porous membrane (3), electrically insulating, and permeable to said liquid medium, placed between the anode (5) and the cathode (7). Said biocell (1) being characterized in that it comprises a means for storing the biofuel (3) and for providing the liquid medium to the anode (5), said means comprising a hydrophilic porous material in contact with said anode (5)) and having a basis weight of 500 to 900 g/m2,

The invention relates to a biocell (1) with a biofuel reservoir intended to be brought into contact with a liquid medium and with a fluid medium comprising an oxidant. Said biocell comprises a first electrochemical cell having: an anode (5) comprising a first enzyme capable of catalyzing the oxidation of the biofuel; —a cathode (7) comprising a second enzyme capable of catalyzing the reduction of the oxidant; and —a separating and porous membrane (3), electrically insulating, and permeable to said liquid medium, placed between the anode (5) and the cathode (7). Said biocell (1) being characterized in that it comprises a means for storing the biofuel (3) and for providing the liquid medium to the anode (5), said means comprising a hydrophilic porous material in contact with said anode (5)) and having a basis weight of 500 to 900 g/m2.

An aspect of the present invention provides a method that makes it possible to improve bilirubin oxidase (BOD) activity. The method for improving the BOD activity in accordance with an embodiment of the present invention includes the step of leaving, in a deoxygenated atmosphere, a BOD solution containing a BOD and a specific activity improving agent.

An anode can include: an electrode substrate; a first region of the substrate having a catalyst composition located thereon, wherein the catalyst composition includes an inorganic or metallic catalyst; and a second region of the substrate having an enzyme composition located thereon, wherein the combination of the catalyst composition and enzyme composition converts a fuel reagent to carbon dioxide at neutral pH. The first region and second region can be separate regions. The catalyst of the catalyst composition can include gold nanoparticles. The catalyst can include an inorganic or metallic catalyst selected from vanadium oxide, titanium (III) chloride, Pd(OAc).sub.2, MnO, zeolite, alumina, graphitic carbon, palladium, platinum, gold, ruthenium, rhodium, iridium, or combinations thereof. The catalyst can be nanoparticle, nanorod, nanodot, or combination thereof. The catalyst can have sizes that range from about 10 to 20 nm.

Compositions of peritoneal dialysis solutions and metabolizing enzymes, and their uses to treat disorders associated with elevated levels of metabolites are disclosed. Animal models of hyperoxalemia are also disclosed.

The invention relates to a biocell (1) with a biofuel reservoir intended to be brought into contact with a liquid medium and with a fluid medium comprising an oxidant. Said biocell comprises a first electrochemical cell having: an anode (5) comprising a first enzyme capable of catalyzing the oxidation of the biofuel;—a cathode (7) comprising a second enzyme capable of catalyzing the reduction of the oxidant; and—a separating and porous membrane (3), electrically insulating, and permeable to said liquid medium, placed between the anode (5) and the cathode (7). Said biocell (1) being characterized in that it comprises a means for storing the biofuel (3) and for providing the liquid medium to the anode (5), said means comprising a hydrophilic porous material in contact with said anode (5)) and having a basis weight of 500 to 900 g/m2,