A genetically modified phototrophic cell for in-vivo production of hydrogen. The phototrophic cell has been genetically modified to the effect that a) at least one of the native photosystem I components has been deleted, b) the native hydrogenase has been deleted, and c) at least one fusion protein is expressed, comprising i. a hydrogenase or hydrogenase component and ii. at least one PSI component, with the proviso that the PSI is complemented by expression of the at least one fusion protein, and the hydrogenase component itself, or together with at least one further hydrogenase component expressibly introduced into the cell, has hydrogenase activity.

The present disclosure provides analyte sensors including one or more NAD(P)-dependent enzymes and an internal supply of NAD(P) for the detection of an analyte. The present disclosure further provides methods of using such analyte sensors for detecting one or more analytes present in a biological sample of a subject, and methods of manufacturing said analyte sensors.

The present disclosure provides analyte sensors including one or more NAD(P)-dependent enzymes and an internal supply of NAD(P) for the detection of an analyte. The present disclosure further provides methods of using such analyte sensors for detecting one or more analytes present in a biological sample of a subject, and methods of manufacturing said analyte sensors.

The present disclosure provides analyte sensors including one or more NAD(P)-dependent enzymes and an internal supply of NAD(P) for the detection of an analyte. The present disclosure further provides methods of using such analyte sensors for detecting one or more analytes present in a biological sample of a subject, and methods of manufacturing said analyte sensors.

A genetically modified phototrophic cell for in-vivo production of hydrogen. The phototrophic cell has been genetically modified to the effect that a) at least one of the native photosystem I components has been deleted, b) the native hydrogenase has been deleted, and c) at least one fusion protein is expressed, comprising i. a hydrogenase or hydrogenase component and ii. at least one PSI component, with the proviso that the PSI is complemented by expression of the at least one fusion protein, and the hydrogenase component itself, or together with at least one further hydrogenase component expressibly introduced into the cell, has hydrogenase activity.