Certain embodiments provide a method for preparing a biochemical product (e.g., phenol, catechol, or muconic acid, or a salt thereof). For example, such methods include contacting a recombinant host having two or more recombinant pathways with a fermentable carbon source and growing the recombinant cell for a time sufficient to synthesize the product. In certain embodiments, each recombinant pathway: 1) is capable of producing the same final biochemical product; 2) comprises at least one gene encoding a polypeptide; 3) is derived from a different endogenous metabolite as its immediate precursor; and 4) converges to the same final product or the same intermediate metabolite.

An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.

The objective of the present invention is to provide a microorganism that makes it possible to produce muconic acid from a lignin-derived aromatic compound with sufficient economic efficiency and without depending on the type of lignin, and a method of producing muconic acid using the microorganism. The objective can be achieved by a transformed microorganism wherein the host microorganism is a microorganism of the genus Pseudomonas that has pcaH gene, pcaG gene, catA gene, and catB gene on its chromosome, and that can assimilate an aromatic compound derived from syringyl lignin; and wherein the transformed microorganism lacks at least one gene selected from the group consisting of pcaH gene and pcaG gene on its chromosome, lacks catB gene on its chromosome, and expresses aroY gene inserted; and a method of producing muconic acid using the transformed microorganism and the like.

Provided is a transformant of a microorganism that has improved catechol productivity.

The present invention pertains to a method for producing high value-added compounds from polyethylene terephthalate. More specifically, the present invention demonstrates that a monomeric terephthalic acid obtained from the chemical hydrolysis of polyethylene terephthalate can be converted to high value-added aromatic compounds and aromatic-derived compounds, and ethylene glycol, which is another monomer of polyethylene terephthalate, can be converted to glycolic acid, which is a cosmetic material. The present invention is characterized by recycling polyethylene terephthalate waste into high value-added compounds.

An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.

Herein are disclosed bacterial cells useful for production of 2-fluoro-cis,cis-muconate and derivatives thereof. The disclosure also provides methods and nucleic acid constructs therefor.

Described herein is a PAH-degrading enzyme mixture obtained from a culture of PAH-utilizing microorganisms having been grown in presence of one or more enzyme inducers. Related methods and devices are also described.

Provided is a transformant of a microorganism that has improved catechol productivity.

Provided herein are composition and process for using an electrochemical device for the reduction of the oxidized state of phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide to the reduced state in which unwanted products of the electrochemical reduction are recovered as the oxidized state of the phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide and returned to the electrochemical device for reduction.