Aerobic microbial processes comprise culturing microbes comprising a (per)chlorate respiration pathway in a bioreactor in a bioprocess employing microbial respiration of chloroxyanions as a source of oxygen for the bioprocess, in the absence of external addition of molecular oxygen.

Biomanufacturing hygiene control in batch and fed-batch bioreactors is provided using a bioreactor employing a chlorite/Cld system, wherein the bioreactor comprises an engineered cultured cell expressing a recombinant cytoplasmic chlorite dismutase (cCld) sufficient to increase chlorite resistance of the cell, and chlorite sufficient to substantially inhibit growth of one or more contaminating microorganisms in the bioreactor yet not substantially inhibit growth of the cell.

The present disclosure relates to recombinantly engineered cells that contain a chlorite dismutase polypeptide and methods for culturing such cells in a culture medium containing chlorite in an amount sufficient to reduce the growth rate or kill contaminating microorganisms without killing the recombinantly engineered cells. Also provided are methods for the production of a fermentation product using the recombinantly engineered cells.

The present disclosure relates to recombinantly engineered cells that contain a chlorite dismutase polypeptide and methods for culturing such cells in a culture medium containing chlorite in an amount sufficient to reduce the growth rate or kill contaminating microorganisms without killing the recombinantly engineered cells. Also provided are methods for the production of a fermentation product using the recombinantly engineered cells.

Biomanufacturing hygiene control in batch and fed-batch bioreactors is provided using a bioreactor employing a chlorite/Cld system, wherein the bioreactor comprises an engineered cultured cell expressing a recombinant cytoplasmic chlorite dismutase (cCld) sufficient to increase chlorite resistance of the cell, and chlorite sufficient to substantially inhibit growth of one or more contaminating microorganisms in the bioreactor yet not substantially inhibit growth of the cell.

Described herein are compositions and methods for generating oxygen in living eukaryotic cells, e.g., animal cells, by expressing a Cld enzyme (i.e., chlorite dismutase, chlorite O.sub.2-lyase, chlorite:O2 lyase), optionally in combination with a transporter, in the cells.

Aspects of the disclosure include the manufacture of non-naturally occurring bacteria for perchlorate bioremediation. An exemplary method includes determining a source bacteria having one or more source genes which code for perchlorate reduction and chlorite dismutase and determining a target bacteria that does not naturally include the source genes. A first sequence of deoxyribonucleic acid (DNA) is identified in the source genes that codes, in the source bacteria, for an ordered set of amino acids whose linear sequence results in a protein which breaks down perchlorates and a second sequence of DNA is determined which, if inserted into the target bacteria, would allow the target bacteria to code for the ordered set of amino acids. A gene package is built by replacing the first sequence of DNA with the second sequence of DNA and the gene package is inserted into the target bacteria, thereby forming a non-naturally occurring modified bacteria.