C12Y113/11058

9-LOX5 GENE VARIANT PROVIDING POWDERY MILDEW RESISTANCE

The present invention relates to a plant with a copy number variation (CNV) of a 9-LOX5 gene, wherein the CNV has at least 2 copies of a 9-LOX5 gene, wherein the 9-LOX5 gene comprises SEQ ID NO: 1, or a sequence having at least 60% sequence identity to SEQ ID NO: 1, and wherein the presence of the CNV leads to powdery mildew resistance in the plant. The invention further relates to nucleic acid molecules, methods for selecting powdery mildew resistant plants. The plants thus obtained and seeds and parts thereof.

Nucleotide sequence encoding 9-lipoxygenase and recombinant constructs comprising the same

The present invention provides a polynucleotide having the nucleotide sequence as set forth in SEQ ID NO: 1 encoding 9-lipoxygenase. The present invention also provides recombinant plasmid expression vector comprising said polynucleotide. The recombinant protein 9-lipoxygenase encoded by the polynucleotide leads to production of lactones in fruits such as mangoes.

NUCLEOTIDE SEQUENCE ENCODING 9-LIPOXYGENASE AND RECOMBINANT CONSTRUCTS COMPRISING THE SAME

The present invention provides a polynucleotide having the nucleotide sequence as set forth in SEQ ID NO: 1 encoding 9-lipoxygenase. The present invention also provides recombinant plasmid expression vector comprising said polynucleotide. The recombinant protein 9-lipoxygenase encoded by the polynucleotide leads to production of lactones in fruits such as mangoes.

Detergent composition

A liquid detergent composition, preferably a liquid manual dishwashing detergent composition, comprising one or more hydroperoxy fatty acid producing enzymes selected from the group consisting of: arachidonate lipoxygenases, alpha-dioxygenases, and mixtures thereof, preferably alpha-dioxygenases, a surfactant system and a liquid carrier (i.e., water). Methods of washing comprising the liquid detergent composition are also provided.

DETERGENT COMPOSITION

A detergent composition, preferably a manual dishwashing detergent composition, including one or more diol synthases capable of converting one or more unsaturated fatty acids into one or more oxylipins, and a surfactant system including one or more anionic surfactants and one or more co-surfactants selected from the group consisting of amphoteric surfactant, zwitterionic surfactant, and mixtures thereof. Method of using the detergent composition including a surfactant system and the diol synthases are also provided.

DETERGENT COMPOSITION

A detergent composition, preferably a manual dishwashing detergent composition, including one or more diol synthases capable of converting one or more unsaturated fatty acids into one or more oxylipins, and a surfactant system including one or more anionic surfactants and one or more co-surfactants selected from the group consisting of amphoteric surfactant, zwitterionic surfactant, and mixtures thereof. Method of using the detergent composition including a surfactant system and the diol synthases are also provided.

DETERGENT COMPOSITION

A liquid detergent composition, preferably a liquid manual dishwashing detergent composition, comprising one or more hydroperoxy fatty acid producing enzymes selected from the group consisting of: arachidonate lipoxygenases, alpha-dioxygenases, and mixtures thereof, preferably alpha-dioxygenases, a surfactant system and a liquid carrier (i.e., water). Methods of washing comprising the liquid detergent composition are also provided.

DETERGENT COMPOSITION

A surfactant containing detergent composition, preferably a manual dishwashing detergent composition, comprising either a combination of one or more hydroperoxy fatty acid producing enzymes and one or more hydroperoxy fatty acid converting enzymes, or at least one fatty acid processing fusion enzyme comprising at least two catalytic domains: a hydroperoxy fatty acid producing domain and a hydroperoxy fatty acid converting, and methods of using such compositions.

9-LOX5 gene variant providing powdery mildew resistance

The present invention relates to a plant with a copy number variation (CNV) of a 9-LOX5 gene, wherein the CNV has at least 2 copies of a 9-LOX5 gene, wherein the 9-LOX5 gene comprises SEQ ID NO: 1, or a sequence having at least 60% sequence identity to SEQ ID NO: 1, and wherein the presence of the CNV leads to powdery mildew resistance in the plant. The invention further relates to nucleic acid molecules, methods for selecting powdery mildew resistant plants, the plants thus obtained therefrom, and seeds and parts thereof.