The present invention relates generally to production methods, enzymes and recombinant yeast strains for the biosynthesis of clinically important prenylated polyketides of the cannabinoid family. Using readily available starting materials, heterologous enzymes are used to direct cannabinoid biosynthesis in yeast.

Herein are disclosed bacterial cells useful for production of 2-fluoro-cis,cis-muconate and derivatives thereof. The disclosure also provides methods and nucleic acid constructs therefor.