The present disclosure provides methods for biosynthesis of acetaminophen. The present disclosure provides host cells genetically modified to provide for production of acetaminophen. The present disclosure provides a recombinant host cell that is genetically modified with one or more heterologous nucleic acids comprising nucleotide sequences encoding 4-aminobenzoate hydroxylase (4ABH) and N-hydroxyarylamine O-acetyltransferase (NhoA). The present disclosure provides a recombinant prokaryotic host cell that is genetically modified with one or more heterologous nucleic acids comprising nucleotide sequences encoding 44ABH and NhoA.

The disclosure relates to a metabolic transistor in microbes such as bacteria and yeast where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the microbe.

The present disclosure provides compositions and methods for the biosynthetic production of acetaminophen, p-aminophenol, and p-aminobenzoic acid and the purification of biologically derived acetaminophen.

The disclosure relates to a metabolic transistor in microbes such as bacteria and yeast where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the microbe.