Described herein are devices and methods for using the same to produce carotenoids. The carotenoids produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods. The devices and methods disclosed herein also enhance one or more physical properties of plants treated with the devices described herein.

Provided herein are recombinant nucleic acid molecules, nucleic acid constructs, fusion enzymes, transformed host cells, and methods for making aroma compounds alpha-ionone or beta-ionone.

Described herein are devices and methods for enhancing the physiological properties of plants. For example, the devices and methods described herein increase the production of lycopene, which has industrial and economic value. The lycopene produced by the devices and methods does not require the ultra purification that is common in conventional or commercial methods. The devices and methods described herein also enhance the growth rate of plants.

This disclosure describes modified photosynthetic microorganisms, including Cyanobacteria that produce carotenoids, including zeaxanthin, astaxanthin, and/or canthaxanthin. The modifications include one or more genetic modifications such as gene deletion, up regulation of an endogenous gene, and/or addition of an exogenous gene. In some embodiments the modified photosynthetic microorganisms may be subjected to stress conditions.

A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway is disclosed. The polynucleotides include: (i) a polynucleotide which encodes Phytoene dehydrogenase (crtI) and a first transcriptional regulatory sequence; (ii) a polynucleotide which encodes Beta-lycopene cyclase (lcy-B) and a second transcriptional regulatory sequence; (iii) a polynucleotide which encodes Beta-carotene ketolase (crtW) and a third transcriptional regulatory sequence; and wherein the first, second and third regulatory sequence are selected such that the expression of the Icy-B and the crtW is greater than a level of expression of the crtI. Methods of generating astaxanthin using the plurality of polynucleotide are also disclosed as well as bacterial cells comprising high levels of astaxanthin.

The present invention provides a method of producing astaxanthin and lysine in recombinant gram-positive bacteria comprising a nucleic acid sequence encoding for a crtZ-protein from F. pelagi and comprises a nucleic acid sequence encoding for a crtW-protein.

Described herein are devices and methods for using the same to produce carotenoids. The carotenoids produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods. The devices and methods disclosed herein also enhance one or more physical properties of plants treated with the devices described herein.

The present invention provides a method of producing astaxanthin and lysine in recombinant gram-positive bacteria comprising a nucleic acid sequence encoding for a crtZ-protein from F. pelagi and comprises a nucleic acid sequence encoding for a crtW-protein.

Provided is a mutant having an ability to overproduce carotenoids and a method for producing carotenoids by using the mutant. The mutant, of which mutations are induced by irradiation after being transformed with a recombinant vector according to the subject matter, has an excellent ability to produce carotenoids and can be mass-produced, and thus can be useful in various industrial fields, which use carotenoids, such as cosmetics, food, and feed.

The present invention relates to a recombinant Deinococcus bacterium comprising a heterologous biosynthetic pathway converting lycopene to astaxanthin or a precursor thereof, and its use for producing astaxanthin or a precursor thereof. Shown is the production of carotene, zeaxanthin, canthaxanthin and astaxanthin using recombinant Deinococcus expressing genes encoding for lycopene cylase (EC 5.5.1.19), beta-carotene hydroxylase (EC1.14.13.129) and beta-carotene ketolase, namely carotenoid-4,4-beta-ionone ring oxygenase (EC 1.14.11.B16).