The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ω3 destaurases, Δ5 elongases and Δ6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.

The present invention provides isolated FAD2, FAD3, FAB1 and FAE1 genes and FAD2, FAD3, FAB1 and FAE1 protein sequences of Camelina species, e.g., Camelina sativa, mutations in Camelina FAD2, FAD3, FAB1 and FAE1 genes, and methods of using the same. In addition, methods of altering Camelina seed composition and/or improving Camelina seed oil quality are disclosed. Furthermore, methods of breeding Camelina cultivars to produce plants having altered or improved seed oil and/or meal quality are provided.

The present disclosure relates to methods for production of 4-hydroxytryptamine and derivatives thereof in a yeast cell. Herein are also disclosed methods for production of halogenated tryptophans and derivatives thereof in a cell. Herein are also disclosed methods for production of methylated tryptamine. The disclosure also provides nucleic acid constructs and cells useful for performing the present methods.

Among the various aspects of the present disclosure relates to methods and compositions useful in convert inflammatory dietary fatty acids (omega 6) to anti-inflammatory fatty acids (omega 3). Specifically, the present disclosure provides methods and compositions for increasing n-3 desaturase expression or activity in a subject. The disclosure provides various compositions for providing increased n-3 desaturase expression or activity including a variety of viral vector and engineered microorganisms The disclosure provides, in part, methods for reducing local and/or systemic inflammation, improving wound healing, preventing premature cellular enescence, treating or preventing a disease, disorder, or condition related to inflammation or obesity and treating or preventing arthritis.

This disclosure concerns the metabolic engineering of microorganisms to provide biosynthetic methods for the production of insect pheromones and precursors thereof in a scalable and eco-friendly fermentation reaction; for example, by converting saturated or unsaturated substrate feedstocks utilizing exogenous metabolic machinery.

T-DNA for expression of a target gene in a plant, wherein the T-DNA comprises a left and a right border element and at least one expression cassette comprising a promoter, operatively linked thereto a target gene, and downstream thereof a terminator, wherein the length of the T-DNA, measured from left to right border element and comprising the target gene, has a length of at least 30000 bp.

The present invention comprises a novel method to engineer soil bacteria to produce powerful chlorinated auxins. While chlorinated auxins were only found in few plant species, this technology will allow the construction of soil bacterial strains capable of producing chlorinated derivatives of indole-3-acetic acid (IAA). A select halogenase can be expressed in soil bacteria by inserting it into the genome or through an expression vector. The engineered strains can then be applied to any plants to promote growth, thus having promising applications in agriculture.

Materials and methods for creating canola (e.g., Brassica napus) lines having oil with increased oleic acid content are provided herein.

A mutant algal strain showing upregulation of mRNA transcripts encoding urea carboxylase, Δ-15-ω3-desaturase and downregulation of mRNA transcripts of gene encoding triacylglycerol lipase is provided herein. The mutant algal strain of the present disclosure is tolerant to low temperature and thus can be grown over a wide temperature range. The strain shows enhanced biomass and fatty acid production and enhanced growth rate and nitrogen metabolism over a wide temperature range of about 10° C. to about 37° C., wherein the enhancement is in comparison to the wild type algal strain. A method of obtaining the mutant algal strain and a method of producing industrially relevant products such as fatty acids from the mutant algal strain also are provided herein.