Disclosed herein are isolated recombinant cells for the expression of 252 nicotinic acetylcholine receptor (nAChR) and methods of use thereof.

Described herein is a biocompatible lipid nanoparticle (LNP) composition suitable for delivering RNA payloads into cells and tissues of a subject. The biocompatible LNPs comprise an ionizable cationic lipid as a core component and have a net neutral surface charge at physiological pH. Delivery of LNP-encapsulated siRNA inhibiting the expression Spermidine/spermine N1-acetyltransferase 1 (SAT1) is shown to inhibit proliferation of a glioblastoma cell line, but not in other cells pertinent to brain tissue such as microvascular endothelial cells, primary human astrocytes, and macrophage cells. Use of a cadherin-binding peptide to increase delivery of LNP-encapsulated siRNA across a blood-brain barrier monolayer model is also described.