The present application provides glycosylation engineered higher eukaryotic cells which produce homogeneous forms of sialylated glycans present on a recombinant glycoprotein. In particular, the glycosylation engineered higher eukaryotic cells produce homogeneous forms of small N-glycan structures which carry terminal sialic acid residues. In addition, the invention provides glycan-conjugates based on specific coupling with sialic acid residues present on the recombinant glycoproteins.

The present application provides glycosylation engineered higher eukaryotic cells which produce homogeneous forms of sialylated glycans present on a recombinant glycoprotein. In particular, the glycosylation engineered higher eukaryotic cells produce homogeneous forms of small N-glycan structures which carry terminal sialic acid residues. In addition, the invention provides glycan-conjugates based on specific coupling with sialic acid residues present on the recombinant glycoproteins.