The invention relates to a plasmid, a DNA assembly method and its application recombinant strain. The plasmid has single adjacent Type IIP and Type IIS RE recognition sites. The plasmid combines the properties of Type IIP and Type IIS REs to achieve recursive cycling, SCAR-free and repeat sequence assembly.

The present disclosure relates to melon plants that are resistant to the plant disease downy mildew caused by the pathogen Pseudoperonospora cubensis. Specifically, the present disclosure relates to melon plants that are resistant to downy mildew disease including a mutated homoserine kinase gene encoding a mutated homoserine kinase protein. The present disclosure further relates to seeds, tissues, cells, or plant parts of the present melon plants, and to methods for identifying downy mildew resistant melon plants.

The invention relates to lettuce plants comprising in its genome a mutant homoserine kinase allele in homozygous form, whereby the plants are resistant against Bremia lactucae.

A method of the bio-production of methionine and/or of its derivatives thereof from a reduced source of sulfur, such as MeSH or MeSNa including genetically modified yeasts, having an increased ability to produce methionine and/or its derivatives thereof, as compared to the parent yeasts.

The present disclosure relates to squash plants that are resistant to the plant disease downy mildew caused by the pathogen Pseudoperonospora cubensis and/or powdery mildew caused by the pathogen Erysiphe cichoracearum. Specifically, the present disclosure relates to squash plants that are resistant to mildew disease including a mutated homoserine kinase gene encoding a mutated homoserine kinase protein. The present disclosure further relates to seeds, tissues, cells, or plant parts of the present squash plants, and to methods for identifying mildew resistant squash plants.

Recombinant microbial cells are provided which have been engineered to produce fatty acid derivatives having linear chains containing an odd number of carbon atoms by the fatty acid biosynthetic pathway. Also provided are methods of making odd chain fatty acid derivatives using the recombinant microbial cells, and compositions comprising odd chain fatty acid derivatives produced by such methods.

Disclosed is a microorganism of Escherichia sp. producing O-acetyl homoserine, and a method of producing O-acetyl homoserine in high yield using the microorganism.

A method of the bio-production of methionine and/or of its derivatives thereof from a reduced source of sulfur, such as MeSH or MeSNa including genetically modified yeasts, having an increased ability to produce methionine and/or its derivatives thereof, as compared to the parent yeasts.

The invention relates to a plasmid, a DNA assembly method and its application recombinant strain. The plasmid has single adjacent Type IIP and Type IIS RE recognition sites. The plasmid combines the properties of Type IIP and Type IIS REs to achieve recursive cycling, SCAR-free and repeat sequence assembly.

Disclosed is a microorganism of Escherichia sp. producing O-acetyl homoserine, and a method of producing O-acetyl homoserine in high yield using the microorganism.