The present invention relates to a novel Chlamydomonas strain with an improved oil generation function, the strain of the present invention having useful mycological characteristics as a strain that provides a useful substance, such as a vegetable oil, in a microalga, as the strain has a fast cell growth speed and an excellent lipid generation function compared to conventional strains. In particular, the present invention can provide a vegetable oil with improved stability and a longer preservation period by containing, in a cell, a large amount of antioxidant pigments such as lutein and zeaxanthin, and can, thereby, be usefully used in industries such as food, medicine, cosmetics, etc., which utilize a vegetable oil.

The present disclosure provides methanotrophic bacteria that are modified to produce less glycogen, and methods of using the modified methanotrophic bacteria to produce a desired product, such as protein(s) or metabolite(s).

A recombinant microorganism for producing 2,3-butanediol consisting of selecting at least three groups from uridine diphosphate glucose phosphate uroglycan transferase gene (galU), acetyl alcohol dehydrogenase gene (acoA), acetyl phosphate transferase gene (pta), adenosine glucosylphosphate transferase gene (glgC), lactose dehydrogenase gene (ldhA), and phosphodiesterase gene (pdeC) which were modified.

Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding modified AGPase large subunit proteins, polypeptides encompassing modified AGPase large subunit proteins, methods of producing modified polynucleotides encoding modified AGPase large subunit proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing plants comprising the modified AGPase large subunit genes of the invention or encoding the AGPase large subunit proteins of the invention are also provided. Compositions and methods for the ready detection of precise base changes are also provided herein. Compositions include primer pads as part of a repair donor template, and methods for the detection of precise base changes include PCR detection using one or more primers designed to anneal with a primer pad sequence.

Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding ADP-glucose pyrophosphorylase small subunit (AGPaseSS) proteins, polypeptides encompassing AGPaseSS proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.

The present invention discloses a preparation method of a synthetase for continuous monitoring of blood glucose. The method includes the preparation steps of step S1: hydroxylation of a glass bead; step S2: silanization of the glass bead; step S3: bonding of the glass bead to glucose; step S4: synthesis of a polymer particle on the surface of the glass bead to form a glucose synthetase; and step S5: separation of the highly specific glucose synthetase. According to the preparation method of a synthetase for continuous monitoring of blood glucose provided by the present invention, the glass bead is bonded to the glucose by activating the surface of the glass bead, and then bonded to a polymer to form the glucose synthetase. The artificial synthetase prepared by the method has low sensitivity to temperature and pH value, and can be directly used in a blood glucose electrochemical sensor.

Recombinant microorganisms configured for increased glycogen production. The recombinant microorganisms comprise a recombinant nucleic acid configured to express or overexpress a glucose-1-phosphate adenylyltransferase. The recombinant microorganisms produce an increased amount of glycogen compared to a corresponding microorganism not comprising the recombinant nucleic acid.

The invention relates to genetically modified agricultural plants with increased oil content in vegetative tissues, as well as to expression systems, plant cells, seeds and vegetative tissues related thereto.

Recombinant microorganisms configured for increased glycogen production. The recombinant microorganisms comprise a recombinant nucleic acid configured to express or overexpress a glucose-1-phosphate adenylyltransferase. The recombinant microorganisms produce an increased amount of glycogen compared to a corresponding microorganism not comprising the recombinant nucleic acid.

Recombinant microorganisms configured for increased glycogen production. The recombinant microorganisms comprise a recombinant nucleic acid configured to express or overexpress a glucose-1-phosphate adenylyltransferase. The recombinant microorganisms produce an increased amount of glycogen compared to a corresponding microorganism not comprising the recombinant nucleic acid.