Compositions comprising an engineered or non-naturally occurring polypeptides comprising two or more domains selected from Sulfide quinone oxidoreductase, Thiosulfate Sulfurtransferase and persulfide dioxygenase, and a phosphate binding motif are provided along with methods of detoxifying bacterial endotoxins and H.sub.2S with such compositions.

Compositions comprising a rhodanese with a phosphate-binding motif and methods of detoxifying bacterial endotoxins with such compositions.

Compositions comprising a rhodanese with a phosphate-binding motif and methods of detoxifying bacterial endotoxins with such compositions.

Compositions comprising a rhodanese with a phosphate-binding motif and methods of detoxifying bacterial endotoxins with such compositions.

A compound enzyme, comprising L-histidine methylase, halogen methyltransferase and trimethylhistidine sulfurylase. L-histidine is catalyzed by L-histidine methylase and halogen methyltransferase to obtain trimethylhistidine, and then is catalyzed by trimethylhistidine sulfurase to obtain the L-ergothioneine. The method can realize conversion from L-histidine to L-ergothioneine only in two steps. Moreover, SAM enzyme is regenerated, such that the usage amount of the SAM enzyme is greatly reduced. Therefore, the method greatly reduces the raw material cost. Moreover, the method is high in reaction concentration (about 30 g/L), simple in process and good in industrial production prospect.

The present disclosure generally relates to technologies for the degradation a systemic toxin in vivo by enhancing the metabolic functionality of the lungs. Such technologies comprise introducing, to pulmonary tissue of a subject, a composition comprising at least one enzyme known to enzymatically break down at least one toxin present systemically in the subject. The provided technologies enable the lung to behave as a tunable metabolic organ, facilitating the removal of toxins from the systemic circulation of a subject, such as a subject having a condition resulting in the toxic accumulation or an endogenous metabolite, and a subject who has consumed a toxic substance.

The invention relates to a process for the fermentative preparation of sulphur-containing amino acids chosen from the group of L-methionine, L-cysteine, L-cystine, L-homocysteine and L-homocystine, comprising the steps: a) provision of a microorganism of the family Enterobacteriaceae or of a microorganism of the family Corynebacteriaceae which has an increased thiosulphate sulphurtransferase activity compared with the particular starting strain; b) fermentation of the microorganism, from a) in a medium which contains an inorganic source of sulphur chosen from the group of salt of dithiosulphuric acid or a mixture of a salt, of dithiosulphuric acid and a salt of sulphuric acid, a fermentation broth being obtained, and c) concentration of the sulphur-containing amino acid in the fermentation broth from b).