A Gypsophila paniculata plant comprising an exogenous nucleic acid sequence encoding PAP1 is provided. Alternatively or additionally there is provided a Gypsophila paniculata plant comprising a flower producing a non-thermally induced red, pink, purple or green pigmentation or a combination of same.

A composition for wound healing, comprising an amount of a macrophage proresolving polarizer, wherein the amount is effective to promote wound healing. The composition includes wherein the macrophage proresolving polarizer is lipin-1. The composition includes wherein the composition includes one, two, or three of IL-4, apoptotic cells (ACs), and AC derived lipids. The composition includes wherein the macrophage proresolving polarizer is a lipin-1 transcriptional coregulatory activity promoter. The composition includes, wherein the lipin-1 transcriptional coregulatory activity promoter is an inhibitor of lipin-1 macrophage pro-inflammatory responses enzymatic activity. A method for promoting wound healing, comprising contacting a wound on a skin of a mammal with the composition. A kit comprising one or more containers including the composition in sterile packaging. A wound healing device comprising a substrate and an amount of an amount of a macrophage proresolving polarizer, wherein the amount is effective to promote wound healing.

A method of promoting axonal regeneration can include directing neuronal lipid synthesis away from triglyceride synthesis and toward phospholipid synthesis. The method can include administering to the patient a therapeutically effective amount of an inhibitor compound selected from the group consisting of a Lipin-1 inhibitor, a diglyceride acyltransferase inhibitor, and combinations thereof or administering a gene editing therapy to the patient that reduces expression of LIPIN1 or a diglyceride acyltransferase gene.

A method for producing an objective substance such as phytosphingosine (PHS) and phytoceramide (PHC) using yeast is provided. The objective substance is produced by cultivating yeast having an ability to produce the objective substance and modified so that the expression and/or activity of a protein(s) encoded by NEM1 and/or SPO7 gene(s) is reduced in a culture medium.

Methods for treatment of Duchenne's muscular dystrophy and several clinically related conditions in subjects in need thereof are provided. In further aspects, methods are provided for decreasing muscle degeneration in a subject in need thereof. In even further aspects, methods of enhancing sarcolemma stability and or/integrity in muscle cells lacking functional dystrophin in a subject in need thereof are provided. In aspects, the above-methods comprise administering a therapeutically effective amount of a composition suitable for increasing expression of a LPIN1 gene or a lipin-1 protein levels in a muscle cell of said subject; and decreasing muscle degeneration and improving exercise endurance and muscle contractile force in said subject by said step of administering.

Disclosed are nucleotide sequences and corresponding amino acid sequences of Arxula adeninivorans genes that can be utilized to manipulate the lipid content and/or composition of a cell. Methods and compositions for utilizing this information are disclosed to increase the lipid content or modify the lipid composition of a cell by either increasing or decreasing the activity of certain genetic targets.

The present invention discloses a production method of enzymatic reaction using adenosine instead of ATP. The method comprises the following steps: (1) adding ATP regeneration enzyme, AK enzyme and adenosine in proportion to carry out an enzymatic reaction in an enzymatic reaction system; (2) separating the ATP regeneration enzyme and AK enzyme by either directly separating ATP regeneration enzyme and AK enzyme immobilized in a reaction tank or separating free ATP regeneration enzyme and AK enzyme by an ultrafiltration membrane in a filter; and (3) separating and purifying the filtrate of step (2) to obtain a product. The disclosed method provides: greatly reduced industrial production costs; faster reaction rate; stable enzyme recovery system that is energy efficient and environmentally friendly; and capability of reusing the byproducts or collecting them for the production of ATP.

The present invention relates to the production of hydrolyzates from a lignocellulose-containing material, and to fermentation of the hydrolyzates. More specifically, the present invention relates to the detoxification of phenolic inhibitors and toxins formed during the processing of lignocellulose-containing material by enzymatically sulfating the phenolic inhibitors and toxins using aryl sulfotranseferases.

The present invention discloses a production method of enzymatic reaction using adenosine instead of ATP. The method comprises the following steps: (1) adding ATP regeneration enzyme, AK enzyme and adenosine in proportion to carry out an enzymatic reaction in an enzymatic reaction system; (2) separating the ATP regeneration enzyme and AK enzyme by either directly separating ATP regeneration enzyme and AK enzyme immobilized in a reaction tank or separating free ATP regeneration enzyme and AK enzyme by an ultrafiltration membrane in a filter; and (3) separating and purifying the filtrate of step (2) to obtain a product. The disclosed method provides: greatly reduced industrial production costs; faster reaction rate; stable enzyme recovery system that is energy efficient and environmentally friendly; and capability of reusing the byproducts or collecting them for the production of ATP.

The technology described herein is directed to engineered chemoautotrophic bacteria and methods of producing triacylglycerides. Also described herein are systems or bioreactors comprising said engineered bacteria.