Provided are a Blumea balsamifera monoterpene synthase BbTPS3 and related biological materials thereof and use thereof. BbTPS3 is: A1) a protein having the amino acid sequence shown in SEQ ID NO: 2; A2) a fusion protein obtained by linking protein-tags at the N-terminus or/and the C-terminus of the protein shown in SEQ ID NO: 2; and A3) a protein having at least 90% identity and the same function as the protein shown in A1), which is obtained by performing substitution and/or deletion and/or addition of one or more amino acid residues on the amino acid sequence shown in SEQ ID NO: 2. BbTPS3 can catalyze GPP to form l-borneol, and can be used to regulate and produce plant monoterpene compounds and cultivate Blumea balsamifera (L.) DC.

Described herein is a method of producing a drimane sesquiterpene, such as an albicanol compound and/or derivatives thereof, by contacting at least one polypeptide with farnesyl diphosphate (FPP) with a polypeptide of the Haloacid dehalogenase-like (HAD-like) hydrolase superfamily as obtainable from plants of the genus Dryopteris, in particular of the species Dryopteris fragrans. The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. Also described herein are host cells or organisms genetically modified to express the polypeptides and useful to produce a drimane sesquiterpene such as an albicanol compound.

Described herein is a method of producing a drimane sesquiterpene, such as an albicanol compound and/or derivatives thereof, by contacting at least one polypeptide with farnesyl diphosphate (FPP) with a polypeptide of the Haloacid dehalogenase-like (HAD-like) hydrolase superfamily as obtainable from plants of the genus Dryopteris, in particular of the species Dryopteris fragrans. The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. Also described herein are host cells or organisms genetically modified to express the polypeptides and useful to produce a drimane sesquiterpene such as an albicanol compound.

Provided are a Blumea balsamifera monoterpene synthase BbTPS3 and related biological materials thereof and use thereof. BbTPS3 is: A1) a protein having the amino acid sequence shown in SEQ ID NO: 2; A2) a fusion protein obtained by linking protein-tags at the N-terminus or/and the C-terminus of the protein shown in SEQ ID NO: 2; and A3) a protein having at least 90% identity and the same function as the protein shown in A1), which is obtained by performing substitution and/or deletion and/or addition of one or more amino acid residues on the amino acid sequence shown in SEQ ID NO: 2. BbTPS3 can catalyze GPP to form l-borneol, and can be used to regulate and produce plant monoterpene compounds and cultivate Blumea balsamifera (L.) DC.