Provided is a sequencing library which gives reduced sequencing errors, specifically a method for preparing a sequencing library, the method comprising: fragmenting sample DNA; and treating prepared fragments of the sample DNA with a single-strand-specific nuclease to remove single-stranded moieties from the fragments.

Provided herein is a polymerase-free enzyme mix (FRAG) for fragmenting double-stranded DNA. In some embodiments the enzyme mix may comprise a double-stranded DNA nickase and at least one of a DNA ligase capable of sealing a nick within a DNA, and a single-strand specific DNA nuclease. Methods for fragmenting double-stranded DNA are also provided.

Provided herein is a polymerase-free enzyme mix (FRAG) for fragmenting double-stranded DNA. In some embodiments the enzyme mix may comprise a double-stranded DNA nickase and at least one of a DNA ligase capable of sealing a nick within a DNA, and a single-strand specific DNA nuclease. Methods for fragmenting double-stranded DNA are also provided.

This invention relates to in vitro production of nucleic acids, particularly RNAs and specifically messenger RNAs (mRNA).

Provided herein is a polymerase-free enzyme mix (FRAG) for fragmenting double-stranded DNA. In some embodiments the enzyme mix may comprise a double-stranded DNA nickase and at least one of a DNA ligase capable of sealing a nick within a DNA, and a single-strand specific DNA nuclease. Methods for fragmenting double-stranded DNA are also provided.

A kit for preparing a nucleic acid sample for analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) is provided, the kit comprising: a lyophilized enzyme composition comprising: micrococcal nuclease; nuclease P1; and bacterial alkaline phosphatase (BAP); and a digestion buffer. Also provided are enzyme compositions and methods of use for rapid, efficient preparation of a nucleic acid sample for analysis by LC-MS/MS, without the need for denaturation of the sample.

A kit for preparing a nucleic acid sample for analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) is provided, the kit comprising: a lyophilized enzyme composition comprising: micrococcal nuclease; nuclease P1; and bacterial alkaline phosphatase (BAP); and a digestion buffer. Also provided are enzyme compositions and methods of use for rapid, efficient preparation of a nucleic acid sample for analysis by LC-MS/MS, without the need for denaturation of the sample.

A fabric softener composition comprising a quaternary ammonium ester fabric softener compound and an enzyme selected from specific nuclease enzymes, galactanase enzymes and mannanase enzymes. Also, methods of treating a fabric comprising a laundering step, optional rinsing steps and a rinse-treatment step in which the fabric is treated with an aqueous rinse liquor comprising the composition.

A fabric softener composition comprising a quaternary ammonium ester fabric softener compound and an enzyme selected from specific nuclease enzymes, galactanase enzymes and mannanase enzymes. Also, methods of treating a fabric comprising a laundering step, optional rinsing steps and a rinse-treatment step in which the fabric is treated with an aqueous rinse liquor comprising the composition.