The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.

The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.

Provided is a genetically engineered Candida utilis capable of degrading and utilizing kitchen waste. The genetically engineered Candida utilis is obtained by using a Candida utilis multigene co-expression vector to integrate alpha-amylase, glucoamylase and acid protease genes into the Candida utilis genome and to correctly express such three enzymes.

The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.

This invention relates to a medicament or a dietary supplement comprising the Aspergillus niger aspergilloglutamic peptidase that is capable of hydrolyzing plant food allergens, and more particularly, alpha-amylase/trypsin inhibitors, thereby treating diseases due to an innate immune response in humans, and/or allowing to delay the onset of said diseases. The present invention relates to the discovery that the Aspergillus niger aspergilloglutamic peptidase is capable of hydrolyzing alpha-amylase/trypsin inhibitors that are present in wheat and related cereals said inhibitors being strong inducers of innate immune response. Furthermore, the present invention relates to a method for hydrolyzing alpha-amylase/trypsin inhibitors comprising incubating a composition for food consumption comprising alpha-amylase/trypsin inhibitors with the Aspergillus niger aspergilloglutamic peptidase, wherein the inhibitors are hydrolyzed. It also relates to an enzyme composition comprising the Aspergillus niger aspergilloglutamic peptidase and an additional enzyme, and to foodstuff comprising the Aspergillus niger aspergilloglutamic peptidase.

Fungal protease compositions, and more particularly, proteolytic enzyme mixtures comprising a plurality of Aspergillus proteases are provided. The disclosure also relates to protein hydrolysates, food and beverage products and dietary supplements produced using these proteolytic enzyme mixtures, and methods of making and using the same.

Methods of sequencing a protein using a novel digestion-on-emitter technology are provided.

Novel fungal enzyme compositions, and more particularly, enzyme mixtures comprising a plurality of fungal enzymes (e.g., from Aspergillus and Candida) are provided. The disclosure further relates to dietary supplements, foods and beverages containing these enzyme mixtures, and methods of making and using the same.

The present invention provides process for treating crop kernels, comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of an enzyme composition comprising: i) a protease, and ii) a cellulolytic composition, wherein step c) is performed before, during or after step b).

The present invention relates to a novel strain of Trichoderma comprising genetic modifications that enable the improved production of an enzyme cocktail, involving at least upregulation of the transcription factor Xyr1 according to SEQ ID No. 1; disruption of the gene ACE1 according to SEQ ID No. 2; disruption of the gene SLP1 according to SEQ ID No. 3; and expression of the gene Cel3a from Rasamsonia emersonii according to SEQ ID No. 4.