What is described herein relates to a method of selectively hydrolyzing an enantiomer of an alpha haloalkanoic acid according to formula I employing a polypeptide having dehalogenase activity comprising an amino acid sequence as set forth in SEQ ID NO. 1 or SEQ ID NO. 4 or a sequence with at least 80% sequence identity to either of said sequences and to the use of said method.

What is described herein relates to a method of selectively hydrolyzing an enantiomer of an alpha haloalkanoic acid according to formula I employing a polypeptide having dehalogenase activity comprising an amino acid sequence as set forth in SEQ ID NO. 1 or SEQ ID NO. 4 or a sequence with at least 80% sequence identity to either of said sequences and to the use of said method.

Provided are a variant of a haloacid dehalogenase superfamily protein, a polynucleotide encoding the variant, a recombinant microorganism including the variant, and a method of reducing a concentration of a fluorine-containing compound in a sample using the variant.

Provided is a linked polynucleotide in which polynucleotides encoding a promoter, an anchoring motif, and a dehalogenase, respectively, are operatively linked to one another; a host cell including the linked polynucleotide; and a method of reducing a concentration of a fluorine-containing compound in a sample by using the host cell.

Provided are a haloacid dehalogenase superfamily protein variant and a method of reducing a concentration of a fluorine-containing compound in sample using the same.

Provided are a microorganism including a gene encoding a protein having a dehalogenase activity, a composition including the microorganism for use in reducing a concentration of a fluorine-containing compound in a sample, and a method of reducing the concentration of the fluorine-containing compound in the sample by using the microorganism.

Provided are a microorganism having activity in reducing a concentration of a fluorine-containing compound in a sample, a recombinant microorganism including a gene derived from the microorganism, and a method of reducing the concentration of the fluorine-containing compound in the sample by using the microorganism or recombinant microorganism.

Provided is a microorganism including a gene encoding a protein having a dehalogenase activity, a composition for using in reducing a concentration of fluorinated methane in a sample, the composition including the microorganism including the gene encoding the protein having the dehalogenase activity, and a method of reducing the concentration of fluorinated methane in the sample.

An exhaust gas decomposition apparatus or system that includes a bioreactor fluorine-containing compound is decomposed by contact with the first and second fluids in the bioreactor. The first fluid is supplied through a bioreactor inlet and is exhausted through the bioreactor outlet, and moves in a first direction in the bioreactor. The first or second fluid includes a biological catalyst such as an enzyme or recombinant microbe, while the other fluid includes a fluorine-containing compound. As a result, the fluorine-compounds is efficiently biologically remediated by the biological catalyst.

Provided is a recombinant microorganism including a genetic modification that increases a 2-haloacid dehalogenase activity, a method of preparing the recombinant microorganism, and a method of using the recombinant microorganism for reducing the concentration of fluorinated methane in a sample.