The present invention provides for a Pseudomonas cell is able to grow in a medium with xylose or galactose as a sole carbon source with a growth rate of equal to or higher than 0.10 h.sup.−1. The present invention provides for methods and compositions relating to an engineered Pseudomonas putida KT2440 utilizing a non-native carbon source, such as galactose or xylose or both.

The present disclosure provides an Escherichia coli transformant and a method for producing itaconate using the Escherichia coli transformant.

The present disclosure provides an Escherichia coli transformant and a method for producing itaconate using the Escherichia coli transformant.

The present invention provides a transgenic strain for producing succinate. The transgenic strain comprises an autotrophic host cell, and a plurality of exogenous genes within the host cell. The exogenous genes include a gene for expressing -ketoglutarate decarboxylase (Kgd), a gene for expressing succinate semialdehyde dehydrogenase (GabD), a gene for expressing citrate synthase (GltA) and a gene for expressing phosphoenolpyruvate carboxylase (Ppc). Further, expression of at least one of the native genes encoding glucose-1-phosphate adenylyltransferase (GlgC), succinate dehydrogenase subunit A (SdhA), and succinate dehydrogenase subunit B (SdhB) is suppressed in the host cell. The present invention further provides a method for producing succinate, which comprises: providing a transgenic strain of the present invention, and culturing the transgenic strain under a preset condition.

The present disclosure provides methods of modulating the flux of carbon through the monoethylene glycol (MEG) biosynthesis pathway and one or more C3 compound biosynthesis pathways by expressing enzymes that are essential for improving C3 compounds and modulating other genetic aspects of MEG and C3 compound biosynthesis. The disclosure is further drawn to modified microbes comprising the disrupted sequences and overexpressed sequences, and compositions thereof.

Recombinant Zymomonas mobilis for producing ethylene glycol, method and uses thereof are provided. The recombinant Zymomonas mobilis carries and expresses genes related to a synthesis pathway of xylonic acid and genes related to a synthesis pathway of ethylene glycol.

Recombinant Zymomonas mobilis for producing ethylene glycol, method and uses thereof are provided. The recombinant Zymomonas mobilis carries and expresses genes related to a synthesis pathway of xylonic acid and genes related to a synthesis pathway of ethylene glycol.

This disclosure describes a recombinant microbial cells and methods of making and using such recombinant microbial cells. Generally, the recombinant cells may be modified to exhibit increased biosynthesis of a TCA derivative compared to a wild-type control. In some embodiments, the TCA derivative can include 1,4-butanediol. In various embodiments, the microbial cell is a fungal cell or a bacterial cell. In some embodiments, the increased biosynthesis of the TCA derivative can include an increase in xylose dehydrogenase activity, xylonolactonase activity, xylonate dehydratase activity, or 2-keto-3-deoxyaldonic acid dehydratase activity.

This disclosure describes a recombinant microbial cells and methods of making and using such recombinant microbial cells. Generally, the recombinant cells may be modified to exhibit increased biosynthesis of a TCA derivative compared to a wild-type control. In some embodiments, the TCA derivative can include 1,4-butanediol. In various embodiments, the microbial cell is a fungal cell or a bacterial cell. In some embodiments, the increased biosynthesis of the TCA derivative can include an increase in xylose dehydrogenase activity, xylonolactonase activity, xylonate dehydratase activity, or 2-keto-3-deoxyaldonic acid dehydratase activity.