This invention provides compositions and methods for providing high product yield of transgenes expressed in cyanobacteria and microalgae.

This invention provides compositions and methods for providing high product yield of transgenes expressed in cyanobacteria and microalgae.

This invention provides compositions and methods for providing high product yield of transgenes expressed in cyanobacteria and microalgae.

This invention provides compositions and methods for providing high product yield of transgenes expressed in cyanobacteria and microalgae.

Engineered microorganisms are provided that convert gaseous substrates, such as producer gas, into limonene. In some embodiments, limonene is pumped out of the cell via an efflux pump. In some embodiments, limonene, produced as described herein, is converted through catalytic dimerization into jet fuel. Producer gas used in the processes described herein for production of limonene may be derived from sources that include gasification of waste feedstock and/or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.

Engineered microorganisms are provided that convert gaseous substrates, such as producer gas, into limonene. In some embodiments, limonene is pumped out of the cell via an efflux pump. In some embodiments, limonene, produced as described herein, is converted through catalytic dimerization into jet fuel. Producer gas used in the processes described herein for production of limonene may be derived from sources that include gasification of waste feedstock and/or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.

Genetically-encoded volatile synthetic biomarkers and methods for detection of various cancers in a subject are provided. In various aspects, embodiments provide compositions for breath-based cancer detection comprising at least one nucleic acid molecule encoding a synthase that catalyzes production of said volatile organic biomarker. The invention also provides devices, such as an electronic nose device, portable electronic nose device, and/or breath analyzer, for breath-based cancer detection comprising said compositions and at least one analyzer.

This invention provides compositions and methods for providing high product yield of transgenes expressed in cyanobacteria and microalgae.

Engineered microorganisms are provided that convert gaseous substrates, such as producer gas, into limonene. In some embodiments, limonene is pumped out of the cell via an efflux pump. In some embodiments, limonene, produced as described herein, is converted through catalytic dimerization into jet fuel. Producer gas used in the processes described herein for production of limonene may be derived from sources that include gasification of waste feedstock and/or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.

This invention provides compositions and methods for providing high product yield of transgenes expressed in cyanobacteria and microalgae.