The present invention provides a new type of galacto-oligosaccharide, having a mannose residue instead of a glucose residue at the reducing end. The invention also relates to compositions comprising this galacto-oligosaccharide, its preparation and use in nutritional compositions.

The present invention relates to a method for preparing pregabalin by a biological enzyme method. In particular, the method comprises producing pregabalin B and an R-configuration compound C by using a compound A as a raw material under the action of a biological enzyme; performing configuration inversion of the separated and recovered R-configuration compound C under the action of an isomerase to produce an S-configuration compound D; and producing pregabalin B from the compound D under the action of a biological enzyme

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The invention relates to an enzymatic process for the preparation and purification of lactulose starting from lactose using cellobiose-2-epimerase (EC 5.1.3.11) followed by converting lactose to monosaccharides using lactase (EC 3.2.1.108) or to galacto-oligosaccharides using a beta-galactosidase (EC 3.2.1.23) and removing the monosaccharides, thereby resulting in a lactulose product containing, based on carbohydrate weight, at least 90 wt % lactulose, less than 1 wt % lactose, and a weight ratio of lactulose/epilactose.sup.>_10.