The invention provides a method of reducing or eliminating the content of triterpenes and/or triterpenoids of latex or rubber from a laticiferous plant or part thereof, comprising genetically modifying the laticiferous plant or part thereof, such that the activity of at least one oxidosqualene cyclase is reduced or eliminated, compared to the respective wild-type or untreated laticiferous plant or part thereof. The invention further provides a laticiferous plant or part thereof being genetically modified such that the activity of at least one oxidosqualene cyclase is reduced or eliminated, compared to the respective wild-type or untreated laticiferous plant or part thereof, and an RNAi being specifically directed against a mRNA transcript of at least one oxidosqualene cyclase, for use in a method of reducing or eliminating the content of triterpenes and/or triterpenoids of latex or rubber from a laticiferous plant or part thereof.

The present invention relates to a method of increasing the yield of at least one of oxidosqualene, triterpenes and/or triterpenoids in a specifically engineered host cell and a respective host cell as well as to the use of said host cell for manufacturing the at least one of oxidosqualene, triterpenes and/or triterpenoids.

Described in this application are proteins and host cells involved in methods of producing mogrol precursors, mogrol, and/or mogrosides.

Described in this application are proteins and host cells involved in methods of producing mogrol precursors, mogrol, and/or mogrosides.

Described in this application are proteins and host cells involved in methods of producing isoprenoid precursors and/or isoprenoids.

The present invention relates to a method of increasing the yield of at least one of oxidosqualene, triterpenes and/or triterpenoids in a specifically engineered host cell and a respective host cell as well as to the use of said host cell for manufacturing the at least one of oxidosqualene, triterpenes and/or triterpenoids.

The invention provides a method of reducing or eliminating the content of triterpenes and/or triterpenoids of latex or rubber from a laticiferous plant or part thereof, comprising genetically modifying the laticiferous plant or part thereof, such that the activity of at least one oxidosqualene cyclase is reduced or eliminated, compared to the respective wild-type or untreated laticiferous plant or part thereof. The invention further provides a laticiferous plant or part thereof being genetically modified such that the activity of at least one oxidosqualene cyclase is reduced or eliminated, compared to the respective wild-type or untreated laticiferous plant or part thereof, and an RNAi being specifically directed against a mRNA transcript of at least one oxidosqualene cyclase, for use in a method of reducing or eliminating the content of triterpenes and/or triterpenoids of latex or rubber from a laticiferous plant or part thereof.

The present invention relates to an expression cassette encoding a fusion protein comprising a nucleotide sequence encoding an amino acid sequence shown in SEQ ID NO: 1 or a fragment thereof, which directs protein decay, or encoding an amino acid sequence which is at least 60% identical to the amino acid sequence which directs protein decay; and also comprising a nucleotide sequence encoding a protein of interest, wherein the nucleotide sequences are fused together in frame. Further, the present invention relates to a vector comprising the expression cassette, a host cell comprising the expression cassette or a host cell comprising the vector which comprises the expression cassette. Additionally, the present invention relates to a method for the production of a triterpenoid using the host cell comprising the expression cassette of the present invention.