A process for producing a solid material comprising isomaltulose crystals and trehalulose, including: A) contacting an enzyme complex with a sucrose-containing solution; B) isomerizing at least a part of the sucrose to isomaltulose and trehalulose; C) separating off the enzyme complex to obatin a solution including isomaltulose, trehalulose and water; D) partially removing the water by evaporation to obtain a concentrated solution; E) bringing the concentrated solution to a temperature range of 30° C. to 63° C. and subsequently inducing isomaltulose crystallization in this temperature range followed by cooling, thereby obtaining a solid material including isomaltulose crystals and trehalulose.

The present invention relates to a method for producing a composition containing isomaltulose from a substrate containing sucrose comprising the steps of: a) contacting the substrate containing sucrose with a particulate carrier-immobilized sucrose isomerase biomass and b) obtaining a composition containing isomaltulose, characterized in that the median particle size d(0.5) of the carrier-immobilized sucrose isomerase biomass is from 370 to 550 μm. The carrier can be an alginate or a polyvinyl alcohol carrier.

The present disclosure provides solutions to various challenges in health, including health challenges related to high consumption of carbohydrate and/or low consumption of soluble fiber. The present disclosure provides, among other things, bacteria engineered for expression of a fiber-synthesizing enzyme, e.g., for expression of the enzyme in the gut (e.g., in the intestine). In particular embodiments, the present disclosure provides bacteria engineered for expression of a fiber-synthesizing enzyme that consumes carbohydrate in the process of synthesizing fiber, e.g., in the gut. The present disclosure further includes formulations of isolated fiber-synthesizing enzymes for administration to subjects.

Sucrose isomerase is used as a nutritional supplement, or can be mixed in with a powderous food/beverage formulation. When an animal, including a human, consumes sucrose, the sucrose isomerase will act on the sucrose present in the food, and will convert the sucrose to other sugars. This results in lowering of the glycemic index of the food without changing the formulation of the food.

The present invention relates to a method for preparing pregabalin by a biological enzyme method. In particular, the method comprises producing pregabalin B and an R-configuration compound C by using a compound A as a raw material under the action of a biological enzyme; performing configuration inversion of the separated and recovered R-configuration compound C under the action of an isomerase to produce an S-configuration compound D; and producing pregabalin B from the compound D under the action of a biological enzyme

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The invention provides granules comprising A) at least one enzyme selected from at least one of the groups selected from transferases of EC 2, hydrolases of EC 3, lyases of EC 4 and isomerases of EC 5, B) at least one polymer selected from C.sub.1-C.sub.10-alkyl acrylate polymer, C.sub.1-C.sub.10-alkyl methacrylate polymer and C.sub.1-C.sub.10-alkyl acrylate-C.sub.1-C.sub.10-alkyl methacrylate copolymer, preferably C.sub.1-C.sub.10-alkyl acrylate-C.sub.1-C.sub.10-alkyl methacrylate copolymer and C) at least one inorganic carrier material.

The present invention provides a process for production of a solid material containing isomaltulose crystals and trehalulose comprising the process steps A) bringing an enzyme complex which is able to catalyse the reaction of sucrose to isomaltulose and trehalulose into contact with a sucrose-containing solution; B) isomerizing at least some of the sucrose to isomaltulose and trehalulose; C) separating off the enzyme complex to give a solution containing isomaltulose, trehalulose and water; D) partial removal of the water by evaporation, while obtaining a concentrated solution with, based on the total solution, a solid content of 75 wt % to 95 wt %, preferably 80 wt % to 93 wt %, particularly preferably 86 wt % to 92 wt %; E) bringing the concentrated solution to a temperature of 30 C. to 63 C., preferably 45 C. to 62 C., even more preferably 55 C. to 60 C., and subsequent induction of isomaltulose crystallisation in this temperature range followed by cooling while obtaining a solid material containing isomaltulose crystals and trehalulose.

The present invention relates to a method for producing a composition containing isomaltulose from a substrate containing sucrose comprising the steps of: a) contacting the substrate containing sucrose with a particulate carrier-immobilized sucrose isomerase biomass and b) obtaining a composition containing isomaltulose, characterized in that the median particle size d(0.5) of the carrier-immobilized sucrose isomerase biomass is from 370 to 550 m. The carrier can be an alginate or a polyvinyl alcohol carrier.

The present invention relates to a method for producing a composition containing isomaltulose from a substrate containing sucrose comprising the steps of: a) contacting the substrate containing sucrose with a particulate carrier-immobilized sucrose isomerase biomass and b) obtaining a composition containing isomaltulose, characterized in that the median particle size d(0.5) of the carrier-immobilized sucrose isomerase biomass is from 370 to 550 m. The carrier can be an alginate or a polyvinyl alcohol carrier.

The present invention is directed towards genetic modification of native gene encoding for sucrose isomerase and isomaltulose synthase to substantially increase the expression level of these enzymes and use of said enzymes in a process to produce rare disaccharides such as isomaltulose and trehalulose. Also disclosed in the present invention is expression constructs comprising the modified genes and a host cells to express the same.