A method of efficiently producing saccharides having glucose as the major component by inexpensively suppressing the non-productive adsorption of the enzyme to lignin is provided. The method of producing saccharides includes: a first step of preparing a water-soluble protein by adding at least any one of an animal protein and a vegetable protein to an aqueous sodium hydroxide solution or an aqueous calcium hydroxide solution to react with each other; a second step of adding the water-soluble protein to a slurry including a biomass; and a third step of producing saccharides having glucose as a major component by adding a degrading enzyme to the slurry for at least any one of a cellulose or a hemicellulose included in the biomass to be degraded by the degrading enzyme simultaneously with addition of the water-soluble protein to the slurry or after addition of the water-soluble protein.

A method of efficiently producing saccharides having glucose as the major component by inexpensively suppressing the non-productive adsorption of the enzyme to lignin is provided. The method of producing saccharides includes: a first step of preparing a water-soluble protein by adding at least any one of an animal protein and a vegetable protein to an aqueous sodium hydroxide solution or an aqueous calcium hydroxide solution to react with each other; a second step of adding the water-soluble protein to a slurry including a biomass; and a third step of producing saccharides having glucose as a major component by adding a degrading enzyme to the slurry for at least any one of a cellulose or a hemicellulose included in the biomass to be degraded by the degrading enzyme simultaneously with addition of the water-soluble protein to the slurry or after addition of the water-soluble protein.

Various organic molecules, ingredients and compositions are prepared from Stevia rebaudiana plant. The compositions can be used as bulking agents, and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

Various organic molecules, ingredients and compositions are prepared from Stevia rebaudiana plant. The compositions can be used as bulking agents, and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

A method of producing saccharides, in which fluidity of the slurry including biomass can be retained; the used amount of the degrading enzyme can be reduced; and high concentration saccharides can be produced, is provided. In addition, a method of producing ethanol, in which high concentration ethanol can produced, is provided. The method of producing saccharides includes the steps of: adding a cellulose degrading enzyme to a slurry including a biomass; and degrading the cellulose included in the biomass by the degrading enzyme to produce saccharide including glucose as a major component. The concentration of the cellulose is retained at 75 g/L or less in a mixed solution including the slurry, the degrading enzyme, and an adsorption inhibitor inhibiting adsorption of the degrading enzyme to a lignin contained in the biomass.

A hardwood-derived carbohydrate composition is disclosed. The hardwood-derived carbohydrate composition comprises monomeric sugars in an amount of 50-80 weight-% based on the total dry matter content of the carbohydrate composition, wherein the monomeric sugars include monomeric glucose and monomeric xylose, the amount of monomeric xylose in the carbohydrate composition being 40-60 weight-% based on the total dry matter content of the carbohydrate composition, and the weight ratio of monomeric glucose to monomeric xylose being 0.067-0.2. Disclosed is also a method for producing a wood-derived carbohydrate composition.

A method for treating biomass including lignocellulosic polymers. The biomass is treated in a mixture of water with at least one oxidizing agent and steam at a temperature in a range of from about 130° C. to about 220° C. for a period from about 5 seconds to about 10 hours. The pH of the mixture is periodically measured for substantially an entire duration of the treating step. As necessary, based on the measured pH of the mixture, adjusting the pH of the mixture into a range of from about pH 4.5 to about pH 7.5 by adding a base to the mixture.

A method for treating biomass including lignocellulosic polymers. The biomass is treated in a mixture of water with at least one oxidizing agent and steam at a temperature in a range of from about 130° C. to about 220° C. for a period from about 5 seconds to about 10 hours. The pH of the mixture is periodically measured for substantially an entire duration of the treating step. As necessary, based on the measured pH of the mixture, adjusting the pH of the mixture into a range of from about pH 4.5 to about pH 7.5 by adding a base to the mixture.

This disclosure describes processes for using a single cellulosic feedstock or a combination of two or more different cellulosic feedstocks with a starch component to produce a fermented product. The process includes separating the components of the cellulosic feedstocks with fractionation, pretreating a component with wet fractionation with chemicals, hydrolysis and fermenta-tion of the pretreated feedstock(s) to produce cellulosic biofuel. The process may include combining the cellulosic feedstock(s) with other components to a cook and/or a fermentation process, distilling and dehydrating the combined components to produce the biofuel. The process may also include producing a whole stillage stream from the feedstock(s) and mechanically processing the whole stillage stream to produce a high-value protein animal feed.

This disclosure describes processes for using a single cellulosic feedstock or a combination of two or more different cellulosic feedstocks with a starch component to produce a fermented product. The process includes separating the components of the cellulosic feedstocks with fractionation, pretreating a component with wet fractionation with chemicals, hydrolysis and fermenta-tion of the pretreated feedstock(s) to produce cellulosic biofuel. The process may include combining the cellulosic feedstock(s) with other components to a cook and/or a fermentation process, distilling and dehydrating the combined components to produce the biofuel. The process may also include producing a whole stillage stream from the feedstock(s) and mechanically processing the whole stillage stream to produce a high-value protein animal feed.