A micro electrical mechanical system (MEMS) valve is provided. The MEMS valve includes first and second bodies, a medium and a thermal element. The first body defines a first channel and a second channel intersecting the first channel. The second body defines a third channel and is movable within the first channel between first and second positions. When the second body is at the first positions, the second and third channels align and permit flow through the second and third channels. When the second body is at the second positions, the second and third channels misalign and inhibit flow through the second channel. The medium is charged into the first channel at opposite sides of the second body. The thermal element is proximate to the first channel and is operable to cause the medium to drive movements of the second body to the first or the second positions.

Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.

Disclosed are examples of valve systems and methods of operating the respective valve systems. An example valve system may include a valve body, an inlet component, an outlet component and a valve tube. The valve body may include a first void and a second void. The inlet component may be coupled to the first void and the outlet component may be coupled to the second void. The valve tube may include a side port and may be positioned through the valve body and coupled to the first void, the inlet component, the second void, and the outlet component. Other valve system examples may include including a valve body, a first septum, a second septum, a first piston, a second piston and a tube. The disclosed methods describe the interaction of the respective components of the respective valve system example.

A method for forming discrete volumes of aqueous fluid may comprise flowing aqueous fluid into a first conduit from a supply of aqueous fluid and flowing into the first conduit a spacing liquid supplied from a second conduit, the spacing liquid being immiscible with the aqueous fluid. The flowing of the aqueous fluid and the spacing liquid into the first conduit forms discrete volumes of the aqueous fluid, with consecutive discrete volumes of the aqueous fluid separated by the spacing liquid. The method may further comprise transferring the discrete volumes of the aqueous fluid and spacing liquid from the first conduit to a third conduit for processing.

Provided is a centrifuge cassette assembly (800) for separating a fluid and related method of manufacture. The cassette assembly includes a first chamber, a second chamber (308), a fluidic channel (808) creating a fluid connection between the first chamber and the second chamber, at least one molded insertion valve (600, 700) configured to control the flow of fluid in the fluidic channel and a heating element (802) for actuating the at least one molded valve. Further provided are Normally Open Valves (NOVs) (700) and Normally Closed Valves (NCVs) (600) which are capable of insertion into, and which control the fluid flow of, the centrifuge cassette assembly.

Distribution valve comprising: a stationary element comprising a first valve bearing surface, said stationary element comprising a plurality of first fluid ports and at least one second fluid port, each of said fluid ports emerging at said first valve bearing surface and being in fluidic communication with a corresponding conduit provided in said stationary element; a movable element comprising a second valve bearing surface in contact with said first bearing surface, said movable element being arranged to be movable with respect to said stationary element and being arranged to bring at least one of said first ports into fluidic communication with said second port in function of the relative position of said movable element with respect to said stationary element;
characterised in that: said stationary element comprises a mixing chamber in fluidic communication with one of said first fluid ports.

In another embodiment, the mixing chamber can be provided in the movable element rather than in the stationary element.

Self-healing microvalves are described herein. The self-healing microvalve can move from a first position to a second position using an electrical input and use a soft hydraulic assembly to return from the second position to the first position. The electrical input can create an electrostatic attraction, causing the compression of the soft hydraulic assembly and movement of the valve gate to seal the microvalve. The elasticity of the soft hydraulic assembly can then return the self-healing microvalve to the original state, once the electrical input is removed.

A sequencing manifold for the purpose of supplying control and supply services of pre-determined temporal sequences to fluid processing assemblies is provided. The functioning of this sequencing manifold requires that translation be applied to the sequencing ports. Actuator mechanisms may supply such translation as either continuous motion or as a series of stepwise motions. Actuator mechanism can be obtained that rely on only mechanical means without the need for a source of electricity. With such actuators, it becomes feasible to conduct the operations of fluid processing assemblies in remote and primitive locations that lack a source of electricity. One skilled in the mechanical arts can provide various actuator mechanisms to meet these requirements.

A fluidic device can include: a plurality of fluid conduits, each fluid conduit including a first conduit portion separated from a second conduit portion; and at least one transport body that is movably positioned between the first conduit portion and the second conduit portion of each fluid conduit. The at least one transport body can include: at least one port adapted to be aligned with a first conduit portion and a second conduit portion of at least one first conduit so as to fluidly couple the first conduit portion with the second conduit portion; and at least one blocking body portion adapted to be aligned with a first conduit portion and a second conduit portion of at least one second conduit so as to fluidly isolate the first conduit portion from the second conduit portion of the at least one second conduit.

A MEMS multiplexing system including: first and second fluid inputs; and a mixing network. The mixing network including: a first channel to receive the first fluid input; a second channel to receive the second fluid input; a multiplexing valve communicating with the first channel and the second channel, the multiplexing valve to cause the transport of the first fluid into the second channel so as to form a first interleaved fluid downstream from the multiplexing valve in the second channel and to cause the transport of the second fluid into the first channel so as to form a second interleaved fluid downstream from the multiplexing valve in the first channel; and the first channel and the second channel intersecting downstream from the valve so as to force mixing of the first interleaved fluid and the second interleaved fluid.