Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.

Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.

A system and method for transferring beverage such as wine from a source container to a destination container. First and second transfer heads can be configured to sealingly engage with an opening of source and destination containers, respectively. The container opening(s) can have a closure such as a cork or septum and the transfer heads can sealingly engage with the container opening without removing the closure, e.g., by passing one or more needles through the closure. The transfer heads and the source container can be held by a support, and the first transfer head can be mounted for pivotal movement relative to the support, e.g., about a horizontal axis, to accommodate differently sized and/or shaped containers. Pressurized gas, e.g., used to purge and/or pressurize the containers, and beverage flow can be routed from one of the transfer heads to the other.

A system and method for transferring beverage such as wine from a source container to a destination container. First and second transfer heads can be configured to sealingly engage with an opening of source and destination containers, respectively. The container opening(s) can have a closure such as a cork or septum and the transfer heads can sealingly engage with the container opening without removing the closure, e.g., by passing one or more needles through the closure. The transfer heads and the source container can be held by a support, and the first transfer head can be mounted for pivotal movement relative to the support, e.g., about a horizontal axis, to accommodate differently sized and/or shaped containers. Pressurized gas, e.g., used to purge and/or pressurize the containers, and beverage flow can be routed from one of the transfer heads to the other.

Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.

Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.

The present invention provides a nonresident system for depressurizing subsea apparatus and lines comprising a depressurizing tool (5) adapted for being coupled to an ROV interface (6) of a subsea apparatus, wherein the depressurizing tool (5) is coupled to an ROV (4), wherein: the ROV interface (6) comprises a first pipeline (6a) for connection to a first hydrocarbon transport line (8), a second pipeline (6b) for connection to second hydrocarbon transport line (9), and a connection mandrel (6d); and the depressurizing tool (5) comprises a suction line (5a) adapted for being connected to the first pipeline (6a) for connection to the first hydrocarbon transport line; a discharge line (5b) adapted for being connected to the second pipeline (6b) for connection to the second hydrocarbon transport line; a pump (5c); and a connector (5d) adapted for being connected to the connection mandrel (6d) of the ROV interface (6). A method is also provided for depressurizing subsea apparatus and lines, comprising the steps of: removing a blind cap (15) from an ROV interface (6) with aid of an ROV (4); coupling a depressurizing tool (5) to the ROV interface (6) of a subsea apparatus (10); suction and removal of fluid from a first hydrocarbon transport line, wherein the first hydrocarbon transport line comprises hydrate formation; and pressurizing and reinjecting the fluid into a second hydrocarbon transport line.

The present invention provides a nonresident system for depressurizing subsea apparatus and lines comprising a depressurizing tool (5) adapted for being coupled to an ROV interface (6) of a subsea apparatus, wherein the depressurizing tool (5) is coupled to an ROV (4), wherein: the ROV interface (6) comprises a first pipeline (6a) for connection to a first hydrocarbon transport line (8), a second pipeline (6b) for connection to second hydrocarbon transport line (9), and a connection mandrel (6d); and the depressurizing tool (5) comprises a suction line (5a) adapted for being connected to the first pipeline (6a) for connection to the first hydrocarbon transport line; a discharge line (5b) adapted for being connected to the second pipeline (6b) for connection to the second hydrocarbon transport line; a pump (5c); and a connector (5d) adapted for being connected to the connection mandrel (6d) of the ROV interface (6). A method is also provided for depressurizing subsea apparatus and lines, comprising the steps of: removing a blind cap (15) from an ROV interface (6) with aid of an ROV (4); coupling a depressurizing tool (5) to the ROV interface (6) of a subsea apparatus (10); suction and removal of fluid from a first hydrocarbon transport line, wherein the first hydrocarbon transport line comprises hydrate formation; and pressurizing and reinjecting the fluid into a second hydrocarbon transport line.

Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.

Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.