Light detection systems are provided. Aspects of the light detection systems include first and second light receivers in fixed positions relative to each other, a plurality of wavelength separators configured to pass light from the first and second light receivers having a predetermined spectral range, and a plurality of light detection modules. Baseplates having a stage for mounting a light receiver, a plurality of recesses for fixing a plurality of light detection modules in rigid alignment relative to the stage, and a heat dissipation opening positioned within each recess are also provided. In addition, particle analysis systems, methods and kits for practicing the invention are disclosed.

A photonic integrated circuit including an input for receiving input electromagnetic radiation having a bandwidth greater than 60 nm; a spectral splitter splitting the electromagnetic radiation into a plurality of spectral channels; a modulator for modulating an amplitude and a phase of one or more of the spectral channels so as to form modulated outputs; and a spectral recombiner for combining the modulated outputs into a single output port outputting output electromagnetic radiation having the desired output spectral intensity profile shaped by and synthesized from the modulated outputs.

Provided herein are devices, systems, and methods for characterizing a biological sample in vivo or ex vivo in real-time using time-resolved spectroscopy. A light source generates a light pulse or continuous light wave and excites the biological sample, inducing a responsive fluorescent signal. A demultiplexer splits the signal into spectral bands and a time delay is applied to the spectral bands so as to capture data with a detector from multiple spectral bands from a single excitation pulse. The biological sample is characterized by analyzing the fluorescence intensity magnitude and/or decay of the spectral bands. The sample may comprise one or more exogenous or endogenous fluorophore. The device may be a two-piece probe with a detachable, disposable distal end. The systems may combine fluorescence spectroscopy with other optical spectroscopy or imaging modalities. The light pulse may be focused at a single focal point or scanned or patterned across an area.

Provided herein are devices, systems, and methods for characterizing a biological sample in vivo or ex vivo in real-time using time-resolved spectroscopy. A light source generates a light pulse or continuous light wave and excites the biological sample, inducing a responsive fluorescent signal. A demultiplexer splits the signal into spectral bands and a time delay is applied to the spectral bands so as to capture data with a detector from multiple spectral bands from a single excitation pulse. The biological sample is characterized by analyzing the fluorescence intensity magnitude and/or decay of the spectral bands. The sample may comprise one or more exogenous or endogenous fluorophore. The device may be a two-piece probe with a detachable, disposable distal end. The systems may combine fluorescence spectroscopy with other optical spectroscopy or imaging modalities. The light pulse may be focused at a single focal point or scanned or patterned across an area.

An image processing device includes a first substrate, a second substrate, a third substrate provided with a power source circuit, and a casing storing the first substrate, the second substrate, and the third substrate, in which the first substrate has a first surface and a second surface, and is provided between the third substrate and the second substrate in a first direction orthogonal to the first surface, a light receiving element is provided on the second surface, the second substrate has a third surface, a fourth surface, and an opening, the third surface faces the second surface, and the opening is provided to overlap the light receiving element in the first direction, a light emitting element is provided on the fourth surface to surround the opening, and the power source circuit is provided on the third substrate not to overlap the light receiving element in the first direction.

An image processing device includes a first substrate, a second substrate, a third substrate provided with a power source circuit, and a casing storing the first substrate, the second substrate, and the third substrate, in which the first substrate has a first surface and a second surface, and is provided between the third substrate and the second substrate in a first direction orthogonal to the first surface, a light receiving element is provided on the second surface, the second substrate has a third surface, a fourth surface, and an opening, the third surface faces the second surface, and the opening is provided to overlap the light receiving element in the first direction, a light emitting element is provided on the fourth surface to surround the opening, and the power source circuit is provided on the third substrate not to overlap the light receiving element in the first direction.

Provided herein are devices, systems, and methods for characterizing a biological sample in vivo or ex vivo in real-time using time-resolved spectroscopy. A light source generates a light pulse or continuous light wave and excites the biological sample, inducing a responsive fluorescent signal. A demultiplexer splits the signal into spectral bands and a time delay is applied to the spectral bands so as to capture data with a detector from multiple spectral bands from a single excitation pulse. The biological sample is characterized by analyzing the fluorescence intensity magnitude and/or decay of the spectral bands. The sample may comprise one or more exogenous or endogenous fluorophore. The device may be a two-piece probe with a detachable, disposable distal end. The systems may combine fluorescence spectroscopy with other optical spectroscopy or imaging modalities. The light pulse may be focused at a single focal point or scanned or patterned across an area.

Provided herein are devices, systems, and methods for characterizing a biological sample in vivo or ex vivo in real-time using time-resolved spectroscopy. A light source generates a light pulse or continuous light wave and excites the biological sample, inducing a responsive fluorescent signal. A demultiplexer splits the signal into spectral bands and a time delay is applied to the spectral bands so as to capture data with a detector from multiple spectral bands from a single excitation pulse. The biological sample is characterized by analyzing the fluorescence intensity magnitude and/or decay of the spectral bands. The sample may comprise one or more exogenous or endogenous fluorophore. The device may be a two-piece probe with a detachable, disposable distal end. The systems may combine fluorescence spectroscopy with other optical spectroscopy or imaging modalities. The light pulse may be focused at a single focal point or scanned or patterned across an area.

Provided herein are devices, systems, and methods for characterizing a biological sample in vivo or ex vivo in real-time using time-resolved spectroscopy. A light source generates a light pulse or continuous light wave and excites the biological sample, inducing a responsive fluorescent signal. A demultiplexer splits the signal into spectral bands and a time delay is applied to the spectral bands so as to capture data with a detector from multiple spectral bands from a single excitation pulse. The biological sample is characterized by analyzing the fluorescence intensity magnitude and/or decay of the spectral bands. The sample may comprise one or more exogenous or endogenous fluorophore. The device may be a two-piece probe with a detachable, disposable distal end. The systems may combine fluorescence spectroscopy with other optical spectroscopy or imaging modalities. The light pulse may be focused at a single focal point or scanned or patterned across an area.

A photonic integrated circuit including an input for receiving input electromagnetic radiation having a bandwidth greater than 60 nm; a spectral splitter splitting the electromagnetic radiation into a plurality of spectral channels; a modulator for modulating an amplitude and a phase of one or more of the spectral channels so as to form modulated outputs; and a spectral recombiner for combining the modulated outputs into a single output port outputting output electromagnetic radiation having the desired output spectral intensity profile shaped by and synthesized from the modulated outputs.