A method for quantifying an exposure dose for a surface is disclosed. The method may include emitting one or more beams of 222 nm light onto a portion of the surface using one or more far ultraviolet (UV) light sources capable of emitting 222 nm light, the portion of the surface being coated with one or more fluorescent coatings. The method may include capturing images of the portion of the surface. The method may include adjusting one or more image characteristics for the captured images using one or more filtering methods. The method may include generating a histogram of the adjusted images based on the one or more filtering methods. The method may include determining a pixel surface area for the generated histogram. The method may include calculating the exposure dose for the surface based on the generated pixel surface area and a predetermined calibration curve.

An information processing apparatus is configured to generate a color chart used for calibration. The information processing apparatus includes a first determination unit and a generation unit. The first determination unit is configured to determine a type of an additional color material to be added to a fluorescent color material used to generate a fluorescent color material chart as the color chart. The generation unit is configured to generate the fluorescent color material chart in which a plurality of patches in which the additional color material of the type determined by the first determination unit is added to the fluorescent color material, are arranged in a line and at least a bar of a color of a recording medium is arranged as a separator between the plurality of patches.

A method is provided. The method comprises receiving a signal representative of a first combined spectrophotometric response of a substrate and deposited colorant. The first combined spectrophotometric response is indicative of a combined reflectance of the substrate and deposited colorant under a first illumination condition. The method further comprises determining, from the received signal, and from a contribution term indicative of an effect of ultra-violet, UV, light on a combined reflectance of the substrate and deposited colorant, an estimate for a second combined spectrophotometric response of the substrate and deposited colorant under a second illumination condition. The first illumination condition or the second illumination condition is a UV-cut illumination condition.

The wide-angle emission filter includes a base matrix, a photoresist, and a colorant. The base matrix has a flat shape and including a transparent material. The base matrix does not generate fluorescent light or phosphorescent light by an excitation light. The photoresist is disposed in the base matrix. The photoresist is fixed in a solid state through at least one method selected from the group consisting of thermal hardening, photo hardening, and drying. The colorant is disposed in the base matrix and includes light having a predetermined wavelength range. The wide-angle emission filter filters the excitation light regardless of an incident angle of the excitation light.

The present invention relates to methods, computer readable medium and systems for detecting and counting single nucleic acid molecules confined in nanoliter volumes using an unmodified camera, such as a cell phone camera. In particular, it identifies colorimetric amplification-indicator dyes that are compatible with the spectral sensitivity of standard mobile phones. The invention further provides an optimal ratiometric image-process for a selected dye to achieve a readout that is robust to lighting conditions and camera hardware and provides unambiguous quantitative results, even for colorblind users.

In general aspect, a white excitation light generating device can include a fluorescent excitation light source that generates fluorescent excitation light, a first light source that generates first monochromatic light of a first wavelength different from a wavelength of the fluorescent excitation light, a second light source that generates second monochromatic light of a second wavelength different from the wavelength of the fluorescent excitation light and the first wavelength, a spectrophoto sensor that measures tristimulus values of at least one of the fluorescent excitation light, the first monochromatic light, and the second monochromatic light, and a controller that adjusts an output of at least one of the fluorescent excitation light source, the first light source, and the second light source on the basis of the measured tristimulus values.

In general aspect, a white excitation light generating device can include a fluorescent excitation light source that generates fluorescent excitation light, a first light source that generates first monochromatic light of a first wavelength different from a wavelength of the fluorescent excitation light, a second light source that generates second monochromatic light of a second wavelength different from the wavelength of the fluorescent excitation light and the first wavelength, a spectrophoto sensor that measures tristimulus values of at least one of the fluorescent excitation light, the first monochromatic light, and the second monochromatic light, and a controller that adjusts an output of at least one of the fluorescent excitation light source, the first light source, and the second light source on the basis of the measured tristimulus values.

A method for quantifying an exposure dose for a surface is disclosed. The method may include emitting one or more beams of 222 nm light onto a portion of the surface using one or more far ultraviolet (UV) light sources capable of emitting 222 nm light, the portion of the surface being coated with one or more fluorescent coatings. The method may include capturing images of the portion of the surface. The method may include adjusting one or more image characteristics for the captured images using one or more filtering methods. The method may include generating a histogram of the adjusted images based on the one or more filtering methods. The method may include determining a pixel surface area for the generated histogram. The method may include calculating the exposure dose for the surface based on the generated pixel surface area and a predetermined calibration curve.

An optical device for sensing a presence of an analyte in a person is provided. The optical device includes a light source, an optical stack, and a reader. The light source emits a first light having a first wavelength. The optical stack is placed on a skin of the person. The optical stack includes a sensor material and an optical filter. The sensor material emits a second light having a second wavelength when irradiated with the first light. An optical property of the second light is sensitive to the presence of the analyte. The optical filter is disposed on the sensor material and includes a plurality of microlayers numbering at least 10 in total. The optical filter has different first and second transmittances at the respective first and second wavelengths.

A method for evaluating curing in an ink composition comprises depositing an ink composition on the surface of an object via a direct-to-object inkjet printing system to form a film thereon, the ink composition comprising a photoinitiator capable of initiating a free radical polymerization process in the ink composition upon the absorption of light to cure the deposited film and a fluorophore capable of emitting viscosity-dependent fluorescence upon the absorption of light; exposing, in-situ, the deposited film to light generated by a first source of light under conditions which initiate the free radical polymerization process to cure the deposited film; exposing the cured film to light generated by a second source of light under conditions which induce fluorescence emission by the fluorophore in the cured film; measuring the fluorescence emission; and determining a degree of cure in the cured film from the measured fluorescence emission and predetermined calibration data.