The invention provides methods and apparatus comprising a multi-wavelength laser source that uses a single unfocused pulse of a low intensity but high power laser over a large sample area to collect Raman scattered collimated light, which is then Rayleigh filtered and focused using a singlet lens into a stacked fiber bundle connected to a customized spectrograph, which separates the individual spectra from the scattered wavelengths using a hybrid diffraction grating for collection onto spectra-specific sections of an array photodetector to measure spectral intensity and thereby identify one or more compounds in the sample.

Aspects of embodiments pertain to a sensing systems configured to receive scene electromagnetic (EM) radiation comprising a first wavelength (WL1) range and a second wavelength (WL2) range. The sensing system comprises at least one spectral filter configured to filter the received scene EM radiation to obtain EM radiation in the WL1 range and the WL2 ranges; and a self-adaptive electromagnetic (EM) energy attenuating structure. The self-adaptive EM energy attenuating structure may comprise material that includes nanosized particles which are configured such that high intensity EM radiation at the WL1 range incident onto a portion of the self-adaptive EM energy attenuating structure causes interband excitation of one or more electron-hole pairs, thereby enabling intraband transition in the portion of the self-adaptive EM energy attenuating structure by EM radiation in the WL2 range.

An optical detector (1) on an application specific integrated circuit (ASIC) comprises at least one photodiode (5) for receiving incident light and configured to provide at least one diode signal, a modulator (2) configured to provide an AC drive signal and to provide a reference signal associated with the AC drive signal; and a lock-in amplifier (6) configured to receive said at least one diode signal from said at least one photodiode (5) and to receive the reference signal from the modulator (2), and to determine at least one of a phase and an amplitude of said at least one diode signal using the reference signal.

A microplate reader simultaneously obtains Raman measurements from samples contained in non-adjacent wells. At least two Raman probes are positioned perpendicularly above or below the microplate to simultaneously acquire Raman spectra data of the non-adjacent liquid samples. Each probe is coupled to a laser and a spectrometer and includes a lens focusing laser light within the sample and collecting light from the sample for the spectrometer. The spectrometer may include a 2D imaging sensor (sCMOS or CCD) to image light from multiple probes simultaneously, spaced from one another to reduce crosstalk. A positioner moves the microplate plate or probes to acquire data from a different subset of non-adjacent samples, and may also vary laser focus within wells during data acquisition. Multiple fluorescence probes may simultaneously acquire fluorescence data from the same samples, or non-adjacent samples. Probes may be fiber-coupled and positioned within a reaction chamber of a liquid handling system.

An optical device may comprise an array of sensor elements that includes a plurality of pixels and a multispectral filter disposed on the array of sensor elements. The multispectral filter may be configured to pass a first transmission percentage of light of a particular spectral range to a first set of pixels of the plurality of pixels and pass a second transmission percentage of light of the particular spectral range to a second set of pixels of the plurality of pixels.

An example system can include a support and two or more sensor elements mounted to the support. Each sensor element can be electrically connected to a common electrical node and may include: a respective quench resistor connected to a respective internal node; and a respective photodiode (PD) connected to the respective internal node; a differentiating element fed by at least one of the photodiodes; a first readout electrode fed by the common electrical node; and a second readout electrode fed by the differentiating element. The common electrical node may be connected to at least one of the quench resistors or at least one of the photodiodes.

Examples of a spectroscopy probe for performing measurements of Raman spectra, reflectance spectra and fluorescence spectra are disclosed. The integrated spectral probe can comprise one or more light sources to provide a white light illumination to generate reflectance spectra, an excitation light to generate an UV/visible fluorescence spectra and a narrow band NIR excitation to induce Raman spectra. The multiple modalities of spectral measurements can be performed within 2 seconds or less. Examples of methods of operating the integrated spectroscopy probe disclosed.

This is to provide a photometric apparatus improved in measurement precision by improving the state of light incident to a sensor, which photometric apparatus 1 comprises a photometric sensor 30 into which light which is an object to be measured is incident, a signal processing means for processing a sensor output by the photometric sensor, and optical systems 50, 100, 92, 93 and 150 which introduces external light into the photometric sensor, wherein a columnar fiber rod 100 in which a center axis is provided along a direction perpendicular to a light receiving surface of the photometric sensor is provided at a part of the optical system.

An optical device may comprise an array of sensor elements that includes a plurality of pixels and a multispectral filter disposed on the array of sensor elements. The multispectral filter may be configured to pass a first transmission percentage of light of a particular spectral range to a first set of pixels of the plurality of pixels and pass a second transmission percentage of light of the particular spectral range to a second set of pixels of the plurality of pixels.

A determination method includes: obtaining measurement data; selecting 0 or more second wavelengths from a plurality of first wavelengths including at least one of a plurality of measurement wavelengths to generate a plurality of individuals, by using a genetic algorithm; inputting, to a first model learned to reproduce a correct answer label of a target object, the measurement data of the target object belonging to a remaining group and a second spectroscopic spectrum determined by the second wavelength to discriminate a label of the target object belonging to the remaining group, for each of the plurality of individuals; and determining whether or not to use the second wavelength as the wavelength of the spectroscopic spectrum for discrimination based on a rate at which the label is correctly discriminated.