The present invention enables highly accurate analysis when visualizing analysis results in spectral imaging.

An surface analysis method includes: acquiring spectral image data regarding a sample surface with use of a spectral camera; extracting n wavelengths dispersed in a specific wavelength range in the acquired spectral image data, and converting spectrums of the wavelengths in the spectral image data into n-dimensional spatial vectors for each pixel; normalizing the spatial vectors of the pixels; clustering the normalized spatial vectors into a specific number of classifications; and identifying and displaying pixels clustered into the classifications, for each of the classifications.

A Raman spectroscopy method includes acquiring a first spectrum of a sample by performing exposure over a first time period in Raman spectroscopy of the sample, calculating a first Raman signal intensity of the first spectrum acquired, calculating a second time period from the first time period based on the ratio of the first Raman signal intensity calculated to a second Raman signal intensity that is a Raman signal intensity required for the Raman spectroscopy, the second time period being an exposure time period required for acquiring the second Raman signal intensity, and acquiring a second spectrum of the sample by performing exposure over the second time period calculated.

A remote sampling sensor for determining characteristics of a sample includes measurement optics and an insertion probe. The measurement optics are configured to emit light and detect returned light. The insertion probe includes a chamber, the chamber being configured to permit the sample to enter the chamber, an insertion tip at a distal end of the insertion probe, and a retro-reflective optic adjacent the insertion tip. The retro-reflective optic is configured to return the light from the measurement optics through the chamber to the measurement optics. The insertion probe is configured to be remotely located from the measurement optics.

A Raman spectrometer 1 comprising a laser 1001 for illuminating a sample S under investigation, an auto-focusing system for focusing the laser 1001 on the sample S under investigation, and a detector 1010 for detecting Raman spectra emitted in response to illumination by the laser 1001. The auto-focusing system further comprises at least one adjustable focusing element for adjusting the location of the focus of the laser, a determination unit 1012 for determining a selected location for the focus of the laser 1001, and a control unit for adjusting the adjustable focusing element to focus the laser at said selected location determined by the determination unit 1012. The auto-focusing system is arranged under the control of software to enable determination of the selected location for the focus of the laser 1001.

A light detection device includes a Fabry-Perot interference filter, a light detector, a spacer that has a placement surface on which a portion outside a light transmission region in a bottom surface of the interference filter is placed, and an adhesive member that adheres the interference filter and the spacer to each other. Elastic modulus of the adhesive member is smaller than elastic modulus of the spacer. At least a part of a lateral surface of the interference filter is located on the placement surface such that a part of the placement surface of the spacer is disposed outside the lateral surface. The adhesive member is disposed in a corner portion formed by the lateral surface of the interference filter and the part of the placement surface of the spacer and contacts each of the lateral surface and the part of the placement surface.

Light detection systems are provided. Aspects of the light detection systems include first and second light receivers in fixed positions relative to each other, a plurality of wavelength separators configured to pass light from the first and second light receivers having a predetermined spectral range, and a plurality of light detection modules. Baseplates having a stage for mounting a light receiver, a plurality of recesses for fixing a plurality of light detection modules in rigid alignment relative to the stage, and a heat dissipation opening positioned within each recess are also provided. In addition, particle analysis systems, methods and kits for practicing the invention are disclosed.

A protective sheath having a closed end and an open end is sized to receive a hand held spectrometer. The spectrometer can be placed in the sheath to calibrate the spectrometer and to measure samples. In a calibration orientation, an optical head of the spectrometer can be oriented toward the closed end of the sheath where a calibration material is located. In a measurement orientation, the optical head of the spectrometer can be oriented toward the open end of the sheath in order to measure a sample. To change the orientation, the spectrometer can be removed from the sheath container and placed in the sheath container with the calibration orientation or the measurement orientation. Accessory container covers can be provided and placed on the open end of the sheath with samples placed therein in order to provide improved measurements.

A radiation measuring device for measuring electromagnetic radiation originating from an external source. The radiation measuring device includes, a spectrometer, a pyranometer, a pyrgeometer, a diffuser, and a control unit. The spectrometer and a pyranometer are positioned in a sensor zone of a housing of the radiation measuring device. The spectrometer measures visible shortwave radiation and near-infrared shortwave radiation received at the sensor zone. The pyranometer measures shortwave radiation received at the sensor zone. The pyrgeometer is positioned in another sensor zone of the housing and measures longwave radiation received at the other sensor zone. The control unit receives radiation measurements from the spectrometer, pyranometer, and pyrgeometer. A corrected amount of radiation received at the sensor zones of the radiation measuring device is determined from the received radiation measurements. Other embodiments are described and claimed.

A computer-implemented method for visualizing the appearances of at least two materials includes obtaining a first set of appearance attributes, the appearance attributes of the first set being associated with a first material, the first set of appearance attributes comprising measured appearance attributes, obtaining a second set of appearance attributes, the appearance attributes of the second set being associated with a second material; and obtaining a geometric model of at least one virtual object, the geometric model defining a three-dimensional macroscopic surface geometry of the virtual object. The invention is characterized by synthesizing a third set of appearance attributes from the first set of appearance attributes and the second set of appearance attributes and visualizing, using a display device, a scene comprising the at least one virtual object, using the third set of appearance attributes, a comparison set of appearance attributes and the geometric model, a first portion of the at least one virtual object being visualized using the third set of appearance attributes, and a second portion of the at least one virtual object being visualized using the comparison set of appearance attributes, to allow a direct a visual comparison of the first set of appearance attributes as modified by the second set of appearance attributes with the comparison set of appearance attributes.

Embodiments of the invention provide a method and system that allows parameters of a desired target image to be determined from hyperspectral imagery of scene. The parameters may be representative of various aspects of the scene being imaged, particularly representative of physical properties of the scene. For example, in some medical imaging contexts, the property being imaged may be blood perfusion or oxygenation saturation level information per pixel. In one embodiment the parameters are obtained by collecting lower temporal and spatial resolution hyperspectral imagery, and then building a virtual hypercube of the information having a higher spatial resolution using a spatiospectral aware demosaicking process, the virtual hypercube then being used for estimation of the desired parameters at the higher spatial resolution. Alternatively, in another embodiment, instead of building the virtual hypercube and then performing the estimation, a joint demosaicking and parameter estimation operation is performed to obtain the parameters. Various white level and spectral calibration operations may also be performed to improve the results obtained. While establishing functional and technical requirements of an intraoperative system for surgery, we present iHSI system embodiments that allows for real-time wide-field HSI and responsive surgical guidance in a highly constrained operating theatre. Two exemplar embodiments exploiting state-of-the-art industrial HSI cameras, respectively using linescan and snapshot imaging technology, were investigated by performing assessments against established design criteria and ex vivo tissue experiments. We further report the use of one real-time iHSI embodiment during an ethically-approved in-patient clinical feasibility case study as part of a spinal fusion surgery therefore successfully validating our assumptions that our invention can be seamlessly integrated into the operating theatre without interrupting the surgical workflow.