A Raman spectroscopy method includes acquiring a first spectrum of a sample by performing exposure over a first time period in Raman spectroscopy of the sample, calculating a first Raman signal intensity of the first spectrum acquired, calculating a second time period from the first time period based on the ratio of the first Raman signal intensity calculated to a second Raman signal intensity that is a Raman signal intensity required for the Raman spectroscopy, the second time period being an exposure time period required for acquiring the second Raman signal intensity, and acquiring a second spectrum of the sample by performing exposure over the second time period calculated.

A multicolor fluorescence analysis device 11 is for detecting fluorescence emitted, as a result of excitation light irradiation, from a plurality of types of fluorophores included in a sample s, and is provided with an irradiation optical unit 520 for irradiating light emitted from a light source 510 onto a sample s as excitation light, a fluorescence condensation unit 530 having a fluorescence filter 531 that transmits light emitted from the sample s and transmits light of transmission wavelength bands different from the excitation wavelength bands, and a two-dimensional detector 554 that has a plurality of types of transmission filters 556 for transmitting prescribed wavelengths of light and detects the intensity of the light of the prescribed wavelength for each transmission filter 556, and the light emitted from at least two fluorophores from among the plurality of types of fluorophores is detected simultaneously and the fluorophore types are identified accordingly.

Disclosed is a method of acquiring and forming a spectrometry image of a sample including the following steps: e1) acquisition of an initial image, composed of pixels, of an area of the sample and definition of a maximum set of N, 2≤N, measurement positions of spectrometry, each measurement position including a coordinate and an intensity determined on the basis of the pixels; e2) assignment of a classification value to each of the N measurement positions on the basis of deviations, calculated based on an intensity difference and a coordinate difference, between the measurement positions; e3) determination of a group of P, 1≤P≤N, measurement positions as a function of the classification values; e4) successively, for each measurement position of the group, positioning of an excitation beam in the measurement position on the area of the sample, acquisition of a spectrometry measurement and formation of the spectrometry image.

A method for detecting at least one property of a plant product, the method including: directing source light including ultraviolet (UV) light at UV wavelengths and polarized visible and/or near-infrared (VIS/NIR) light at VIS/NIR wavelengths onto a region of the plant product; blocking the polarized VIS/NIR light of the source light, and blocking polarized specular reflection from the region of the plant product, from being transmitted to a visible and/or near-infrared (VIS/NIR) spectrometer; and transmitting a portion of emitted light caused by fluorescence and/or diffuse reflection from the region of the plant product to the visible and/or near-infrared (VIS/NIR) spectrometer.

A system and method for characterization and/or calibration of performance of a multispectral imaging (MSI) system equipping the MSI system for use with a multitude of different fluorescent specimens while being independent on optical characteristics of a specified specimen and providing an integrated system level test for the MSI system. A system and method are adapted to additionally evaluate and express operational parameters performance of the MSI system in terms of standardized units and/or to determine the acceptable detection range of the MSI system.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

System for performing chemical spectroscopy on samples from the scale of nanometers to millimeters or more with a multifunctional platform combining analytical and imaging techniques including dual beam photo-thermal spectroscopy with confocal microscopy, Raman spectroscopy, fluorescence detection, various vacuum analytical techniques and/or mass spectrometry. In embodiments described herein, the light beams of a dual-beam system are used for heating and sensing.

The present relates, in general terms, to a device for monitoring oxidative stress in a sample, a method of making the device and a method of monitoring oxidative stress in a sample thereof.

The invention provides systems for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation and methods for using the same. The system includes a laser source, collection fibers, a demultiplexer and an optical delay device. All references cited herein are incorporated by reference in their entirety as though fully set forth. Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of-ordinary skill in the art in which this invention belongs.

The present invention provides a time-resolved single-photon counting apparatus, including an excitation light source for generating pulsed excitation light, a specimen optics for collecting an optical signal caused by irradiating the pulsed excitation light to a specimen, a photoelectric converter for photoelectrically converting the optical signal to generate an analog single-photon signal, an analog-to-digital (AD) signal converter for sampling the analog single-photon signal to convert the same into a digital single-photon signal, a digital photon-discrimination and timing detector for generating a photon-discrimination signal by discriminating the single-photon property of the digital single-photon signal to count a pulse time point of the digital single-photon signal to generate a delay time signal having delay time information, and a time-signal processor for counting valid single-photon detection events according to the delay time with reference to the photon-discrimination signal.