An apparatus comprising: a body having an aperture dimensioned to receive an air-borne particle of corresponding size; first and second electrodes positioned within the aperture between which a potential difference can be applied; and a measurement circuit configured to measure an electrical property between the first and second electrodes such that the presence of the air-borne particle within the aperture can be detected based on a change in the electrical property when the air-borne particle contacts both the first and second electrodes.

An impedance cytometry device is described along with methods of accurately measuring particle size of particles contained in a fluid that is passed through the impedance cytometry device. The impedance cytometry device includes a substrate, and an electrode arrangement deposited on the substrate in a co-planar fashion. The electrode arrangement includes a drive electrode and a plurality of measurement electrodes located in a same plane as the drive electrode. The plurality of measurement electrodes includes at least two pairs of measurement sub-electrodes, each pair of measurement sub-electrodes including a first measurement sub-electrode positioned adjacent to the drive electrode, and a second measurement sub-electrode separated from the drive electrode by a respective first measurement sub-electrode. The impedance cytometry device may be incorporated into a substrate assembly of an electrowetting on dielectric (EWOD) device, such as in a substrate assembly containing electrowetting drive electrodes or a common reference electrode, or into a microfluidic blood counter device.

An outside opening of each aperture of a plurality of counting chambers for performing particle counting based on the electric resistance method is connected to suction pump through a confluent piping. Liquid supplying part supplies an additional liquid to the counting chamber side after completion of counting of counting chamber, so that the liquid level of sample liquid of counting chamber will not descend to aperture or a predetermined liquid level.

Improved resolution and detection of nanoparticles are achieved when a nanopore connecting liquid compartments in a device running on the Coulter principle is provided with fluid coatings such as lipid walls. Fluid lipid walls are made of a lipid bilayer, and preferably include lipid anchored mobile ligands as part of the lipid bilayer. By varying the nature and concentration of the mobile ligand in the lipid bilayer, multifunctional coatings of lipids are provided.

This disclosure provides an impedance cytometer which includes a carrier that can be attached to a living being, with a biosensor mounted thereto. The bio sensor includes a microfluidic flow channel, formed in the carrier, and an impedance circuit. The microfluidic flow channel accommodates passage of a particle therethrough. The impedance circuit, connected to the microfluidic flow channel, includes a signal generator that produces a high-frequency drive signal applied to the flow channel to produce a biosensor output signal having high-frequency variation resulting from the drive signal and low-frequency variation resulting from impedance variation within the flow channel during the particle's passage. A lock-in amplifier is disposed to (i) amplify the bio sensor output signal, (ii) mix the amplified signal with the drive signal, and (iii) frequency-filter the mixed, amplified signal to output an impedance signal representing the low-frequency impedance variation resulting from the passage of the particle. Embodiments enable wearable, personalized cytometry.

In the particle analyzing apparatus of the present invention, first, an inner space with a negative pressure having a predetermined volume is formed in the cylinder of a syringe device for sucking a sample liquid in the measuring chamber, then, the negative pressure is applied to the measuring chamber, the sample liquid is sucked, and measurement of particle is performed in the measuring flow path. The control device calculates a particle analysis value from the measurement signal obtained by the measurement. The particle analysis value is obtained by the sucking force of the negative pressure and the control device further corrects the particle analysis value based on a standard pressure predetermined for the inner space.

When using an immunological analysis method wherein antigen-antibody reactions are used to form complexes of microparticles and a substance being measured, purifying, then measuring by spectroscopy, and a mass spectrometry method wherein antigen-antibody reactions are used to form complexes of microparticles and a substance being measured, purifying, then measuring with a mass spectrometer, the amount of the complex flowing in the flow path for immunological analysis and the amount of the complex flowing in the flow path for mass spectrometry are unknown, so the substance being measured cannot be accurately quantified even when merging information obtained from immunological analysis and information obtained from mass spectrometry. The invention provides a mechanism for quantifying the complexes after formation of the complexes, on the flow path for mass spectrometry and the flow path for immunological analysis.

The present invention provides methods and systems to combine the capabilities of a hematology analyzer with those of a flow cytometer to yield a far more powerful analytical system than either device alone. In one embodiment, a method of analyzing a cell sample includes receiving a first data generated by an analysis of a first aliquot of the sample on a first particle analyzer having a fluorescence measurement device such as a flow cytometer, detecting at least one unresolved cell population in the first data, and accessing a second data stored on a storage device wherein the second data was previously generated by interrogating a second aliquot of the sample using at least one of a cell volume measurement device and a cell conductivity measurement device in a second particle analyzer such as a hematology analyzer. The unresolved cell population in the first data is then resolved using the second data. Corresponding system embodiments are also disclosed.

According to one embodiment, a particle inspection system includes a voltage driving circuit which applies a driving voltage for a particle inspection to a particle inspection chip, a current-voltage conversion circuit which converts, into a voltage signal, a current signal output from the particle inspection chip when the driving voltage is applied to the particle inspection chip, a detection circuit which detects, based on the voltage signal, whether the sample liquid is introduced into a detection region of the particle inspection chip, and an analysis circuit which analyzes the fine particle, in the sample liquid based on the voltage signal. The voltage driving circuit varies the driving voltage based on the detection result of the detection circuit.

A sensor for particle identification includes a first chamber configured to be filled with an electrolytic solution; a first electrode provided inside the first chamber and configured to be connected to an external power supply for applying a voltage; a second chamber configured to be filled with the electrolytic solution; a second electrode provided inside the second chamber and configured to be connected to the external power supply; a data output configured to output measurement data expressing an ion current generated between the first electrode and the second electrode; a partition separating the first chamber and the second chamber; and a presentation device for providing a unique identifier to an external computer device over a network.