A kit and method for flow cytometry include a liquid dye concentrate for fluorescent staining of virus-size particles with a plurality of fluorogenic dyes in a liquid medium. The liquid dye concentrate includes a plurality of fluorogenic dyes and one or both of (i) the liquid medium comprising a liquid mixture including water and liquid phase organic material and (ii) disaccharide dissolved in the liquid medium.

A process for optically sorting a plurality of particles includes: providing a particle receiver; producing particles; receiving the particles by the particle receiver; receiving a light by the particle receiver; producing a standing wave optical interference pattern in an optical interference site of the particle receiver from the light; subjecting the particles to an optical gradient force from the standing wave optical interference pattern; deflecting the particles into a plurality of deflected paths to form the sorted particles from the particles; and propagating the sorted particles from the optical interference site through the deflected paths to optically sort the particles

An analysis device includes an analysis unit configured to receive scattered light, transmitted light, fluorescence, or electromagnetic waves from an observed object located in a light irradiation region light-irradiated from a light source and analyze the observed object on the basis of a signal extracted on the basis of a time axis of an electrical signal output from a light-receiving unit configured to convert the received light or electromagnetic waves into the electrical signal.

A hydrodynamic focusing device comprises first and second flow channels; a wall at least partially defining an envelopment region connected in-line between the first and second flow channels which collectively define a flow direction extending therethrough; and a chimney comprising a body and a sample fluid inlet, extending from the wall and into the envelopment region. The sample fluid inlet faces at least partially perpendicular to the flow direction in the envelopment region, such that the sample fluid inlet is configured to supply a sample fluid into the envelopment region in a direction that is at least partially perpendicular to the flow direction. The body and the sample fluid inlet each have an elongate profile which has a rounded leading edge facing the first flow channel and opposing long edges connecting the leading and trailing edges and tapered towards the trailing edge.

A field flow fractionator (FFF device) 1 classifies particles in a liquid sample by applying a field to a liquid sample supplied from a sample injection device 5. A detector 6 detects the particles in the liquid sample classified by the FFF device 1. A bypass flow path 8 supplies the liquid sample from the sample injection device 5 to the detector 6 without via the FFF device 1. A rotary valve (flow path switching unit) 4 switches a flow path to guide the liquid sample from the sample injection device 5 to the FFF device 1 or a bypass flow path 8. The bypass flow path 8 is provided with a concentration adjusting device 9 for adjusting the concentration of the liquid sample from the sample injection device 5. In a case where a sample with the same quantity as the sample supplied to the FFF device 1 is supplied to the bypass flow path 8 at the time of analysis, the sample is diluted by the concentration adjusting device 9 such that a detection signal from the detector 6 falls within a dynamic range.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

A counting method includes aggregating particles in a sample by action of first-direction dielectrophoretic force, dispersing the aggregated particles by action of second-direction dielectrophoretic force, which is different from the first-direction dielectrophoretic force, capturing a dispersion image including the dispersed particles, and determining the number of particles on the basis of the dispersion image.

A fine particle measuring device performing fine particle measurement includes a particle probe, a pipe connected to the particle probe, and a particle counter connected to the pipe. A cylindrical pipe is disposed on an outer periphery of the pipe and an air flow path is provided between the pipe and the cylindrical pipe.

This relates to acoustic microfluidic systems that can generate emulsions/droplets or encapsulate particles of interest (including mammalian cells, bacteria cells, or other cells) into droplets upon detection of the particles of interest flowing in a stream of particles. The systems operate on the detect/decide/deflect principle wherein the deflection step, in a single operation, not only deflects particles of interest from a stream of particles but also encapsulates the particles of interest in an emulsion droplet. The microfluidic systems have an abrupt transition in the channel geometry from a shorter channel to a taller channel (i.e., in the shape of a ‘step’) to break the stream of the dispersed phase into a droplet upon acoustic actuation. When there is no acoustic wave present, no droplets/emulsions are generated and the stream of particles proceeds uninterrupted. The rapid actuation and post-actuation recovery employed by the microfluidic systems taught herein ensure that the vast majority of selected particles are properly deflected, that few or no empty droplets are produced, and that total throughput remains high.

Provided is a high efficiency and high sensitivity particle capture type terahertz sensing system. The particle capture type terahertz sensing system includes a sensing substrate to capture particles, and a terahertz sensor to emit terahertz electromagnetic waves to the sensing substrate to sense the particles, wherein the sensing substrate includes a base substrate and a particle capture structure layer formed on the base substrate, the particle capture structure layer includes a plurality of slits for focusing the terahertz electromagnetic waves, the particle capture structure layer captures the particles in the plurality of slits using dielectrophoresis, and an area in which the terahertz electromagnetic waves converge to the plurality of slits matches an area in which the particles are captured in the plurality of slits through the dielectrophoresis.